Chemical Properties of Lipopolysaccharides from Spotted Fever Group Rickettsiae and Their Common Antigenicity with Lipopolysaccharides from Proteus Species

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1993 Oct 1

PMID 8406824

Citations 15

Authors

K Amano

M Fujita

T Suto

Affiliations

Soon will be listed here.

Abstract

The lipopolysaccharides (LPS) isolated from spotted fever group (SFG) rickettsia strains Thai tick typhus TT-118 and Katayama were characterized by chemical analyses, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme-linked immunosorbent assay (ELISA), and immunoblotting. These LPS did not contain heptose, but they contained 3-deoxy-D-manno-octulosonic acid (KDO), glucosamine, quinovosamine, phosphate, ribose, an unknown neutral sugar, and palmitic acid. Resolution of the apparent molecular masses of these LPS by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and staining with silver showed ladder-like bands. In an ELISA, convalescent-phase sera from 10 patients with Japanese spotted fever reacted with LPS from the Katayama strain, and 90% (9 of 10) of these sera also reacted with LPS isolated from Proteus vulgaris OX2. Immunoblotting revealed that the sera reacted with the high-molecular-mass bands of LPS from SFG rickettsiae, in addition to those of OX2 LPS. In an ELISA, immunoglobulin M antibodies from these sera reacted with the O-polysaccharide and lipid A portions of LPS from P. vulgaris OX2. The epitopes common to LPS of SFG rickettsiae and P. vulgaris OX2 may be in the O-polysaccharide and lipid A portions.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Osborn M . STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963; 50:499-506. PMC: 221208. DOI: 10.1073/pnas.50.3.499. View

KARKHANIS Y, Zeltner J, Jackson J, Carlo D . A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978; 85(2):595-601. DOI: 10.1016/0003-2697(78)90260-9. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Tsai C, Frasch C . A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982; 119(1):115-9. DOI: 10.1016/0003-2697(82)90673-x. View

Amano K, Williams J . Peptidoglycan of Legionella pneumophila: apparent resistance to lysozyme hydrolysis correlates with a high degree of peptide cross-linking. J Bacteriol. 1983; 153(1):520-6. PMC: 217401. DOI: 10.1128/jb.153.1.520-526.1983. View

Hitchcock P, Brown T . Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983; 154(1):269-77. PMC: 217456. DOI: 10.1128/jb.154.1.269-277.1983. View

Anacker R, PHILIP R, Williams J, LIST R, Mann R . Biochemical and immunochemical analysis of Rickettsia rickettsii strains of various degrees of virulence. Infect Immun. 1984; 44(3):559-64. PMC: 263624. DOI: 10.1128/iai.44.3.559-564.1984. View

Amano K, Williams J . Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. J Bacteriol. 1984; 160(3):994-1002. PMC: 215808. DOI: 10.1128/jb.160.3.994-1002.1984. View

10.

Mahara F, Koga K, Sawada S, Taniguchi T, Shigemi F, Suto T . [The first report of the rickettsial infections of spotted fever group in Japan: three clinical cases]. Kansenshogaku Zasshi. 1985; 59(11):1165-71. DOI: 10.11150/kansenshogakuzasshi1970.59.1165. View

11.

Amano K, Williams J, Missler S, Reinhold V . Structure and biological relationships of Coxiella burnetii lipopolysaccharides. J Biol Chem. 1987; 262(10):4740-7. View

12.

Uchida T, Tashiro F, Funato T, Kitamura Y . Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol Immunol. 1986; 30(12):1323-6. DOI: 10.1111/j.1348-0421.1986.tb03053.x. View

13.

Amano K, Tamura A, Ohashi N, Urakami H, Kaya S, Fukushi K . Deficiency of peptidoglycan and lipopolysaccharide components in Rickettsia tsutsugamushi. Infect Immun. 1987; 55(9):2290-2. PMC: 260693. DOI: 10.1128/iai.55.9.2290-2292.1987. View

14.

Mizushiri S, Amano K, Fuji S, Fukushi K, Watanabe M . Chemical characterization of lipopolysaccharides from Proteus strains used in Weil-Felix test. Microbiol Immunol. 1990; 34(2):121-33. DOI: 10.1111/j.1348-0421.1990.tb00997.x. View

15.

Amano K, Mizushiri S, Fujii S, Fukushi K, Suto T . Immunological characterization of lipopolysaccharides from Proteus strains used in Weil-Felix test and reactivity with patient sera of tsutsugamushi diseases. Microbiol Immunol. 1990; 34(2):135-45. DOI: 10.1111/j.1348-0421.1990.tb00998.x. View

16.

Vinogradov E, Kaca W, Rozalski A, Shashkov A, Cedzynski M, Knirel Y . Structural and immunochemical studies of O-specific polysaccharide of Proteus vulgaris 5/43 belonging to OX19 group (O-variants). Eur J Biochem. 1991; 200(1):195-201. DOI: 10.1111/j.1432-1033.1991.tb21067.x. View

17.

Amano K, Hatakeyama H, Sasaki Y, Ito R, Tamura A, Suto T . Electron microscopic studies on the in vitro proliferation of spotted fever group rickettsia isolated in Japan. Microbiol Immunol. 1991; 35(8):623-9. DOI: 10.1111/j.1348-0421.1991.tb01594.x. View

18.

Teysseire N, Raoult D . Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever. J Clin Microbiol. 1992; 30(2):455-60. PMC: 265077. DOI: 10.1128/jcm.30.2.455-460.1992. View

19.

Amano K, Suzuki N, Hatakeyama H, Kasahara Y, Fujii S, Fukushi K . The reactivity between rickettsiae and Weil-Felix test antigens against sera of rickettsial disease patients. Acta Virol. 1992; 36(1):67-72. View

20.

Amano K, Hatakeyama H, Okuta M, Suto T, Mahara F . Serological studies of antigenic similarity between Japanese spotted fever rickettsiae and Weil-Felix test antigens. J Clin Microbiol. 1992; 30(9):2441-6. PMC: 265520. DOI: 10.1128/jcm.30.9.2441-2446.1992. View