Molecular Cloning and Expression of a CDNA Encoding a Receptor for Pituitary Adenylate Cyclase Activating Polypeptide (PACAP)

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1993 Aug 23

PMID 8394834

Citations 11

Authors

J A Morrow

E M Lutz

K M West

G Fink

A J Harmar

Affiliations

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Abstract

We have cloned and sequenced a novel cDNA (RPR7) encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP). RPR7 was identified by PCR of rat pituitary cDNA, and full-length clones were isolated from a rat olfactory bulb cDNA library. When expressed in COS cells, RPR7 was functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP) in response to stimulation by PACAP-38, PACAP-27, vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). The order of potency of these ligands was PACAP-38-PACAP-27 > VIP > PHI, suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type I receptor.

Citing Articles

Current State of Understanding of the Role of PACAP in the Hypothalamo-Hypophyseal Gonadotropin Functions of Mammals.

Koves K, Szabo E, Kantor O, Heinzlmann A, Szabo F, Csaki A Front Endocrinol (Lausanne). 2020; 11:88.

PMID: 32210912 PMC: 7067695. DOI: 10.3389/fendo.2020.00088.

Discovery of PACAP and its receptors in the brain.

Hirabayashi T, Nakamachi T, Shioda S J Headache Pain. 2018; 19(1):28.

PMID: 29619773 PMC: 5884755. DOI: 10.1186/s10194-018-0855-1.

Advent and recent advances in research on the role of pituitary adenylate cyclase-activating polypeptide (PACAP) in the regulation of gonadotropic hormone secretion of female rats.

Koves K, Kantor O, Lakatos A, Szabo E, Kirilly E, Heinzlmann A J Mol Neurosci. 2014; 54(3):494-511.

PMID: 24696167 DOI: 10.1007/s12031-014-0294-7.

Agnathan VIP, PACAP and their receptors: ancestral origins of today's highly diversified forms.

Ng S, Chow B, Kasamatsu J, Kasahara M, Lee L PLoS One. 2012; 7(9):e44691.

PMID: 22957100 PMC: 3434177. DOI: 10.1371/journal.pone.0044691.

Neuropeptides of the pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide/growth hormone-releasing hormone/secretin family in testis.

Li M, Arimura A Endocrine. 2003; 20(3):201-14.

PMID: 12721498 DOI: 10.1385/ENDO:20:3:201.