Isolabeling of the Long Arm of the Human Y Chromosome Demonstrated by the FPG Technique

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1978 Oct 20

PMID 83934

Citations 7

Authors

Z Gibas

J Limon

Affiliations

Soon will be listed here.

Abstract

Isolabeling segments were found in the distal region of the long arm of Y chromosomes derived from human leukocytes grown through two replication cycles in medium containing BrdU and stained by the FPG technique. Three main types of Y chromosome staining patterns were demonstrated: I-Y chromosome with typical SCD, II-Y chromosome with weakly stained distal regions of long arms (isolabeling segments), III-Y chromosome with both terminal regions displaying SCD interrupted by one isolabeled segment. The existence of different types of Y chromosome staining patterns was explained on the basis of the previously described hypothesis of unequal distribution of thymine residues between two DNA polynucleotide chains in the distal part of the long arms of human Y chromosomes.

Citing Articles

Replication variants of the human inactive X chromosome. I. Variability within lymphocytes of single individuals.

Schmidt M, Stolzmann W, Baranovskaya L Chromosoma. 1982; 85(3):405-12.

PMID: 7117031 DOI: 10.1007/BF00330363.

A synopsis of the human Y chromosome.

BUHLER E Hum Genet. 1980; 55(2):145-75.

PMID: 7005073 DOI: 10.1007/BF00291764.

Sister chromatid exchange analysis.

LATT S, Schreck R Am J Hum Genet. 1980; 32(3):297-313.

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BrdU-Hoechst-Giemsa analysis of DNA replication in synchronized lymphocyte cultures. Study of human X and Y chromosomes.

Kaluzewski B Chromosoma. 1982; 85(4):553-69.

PMID: 6181944 DOI: 10.1007/BF00327350.

Sister chromatid differentiation and isolabeling of chromosomes.

Ved Brat S, Verma R, Dosik H Hum Genet. 1981; 56(3):305-8.

PMID: 6165669 DOI: 10.1007/BF00274684.

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