Identification of a Novel Sequence Mediating Regulated Endocytosis of the G Protein-coupled Alpha-pheromone Receptor in Yeast

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 1993 May 1

PMID 8392878

Citations 52

Authors

J Rohrer

H Benedetti

B Zanolari

H Riezman

Affiliations

Soon will be listed here.

Abstract

The Saccharomyces cerevisiae alpha-pheromone receptor, a polytopic, G protein-coupled, membrane protein, is internalized after binding of alpha-factor. Mutational analysis suggested that the first 39 residues of the receptor's cytoplasmic tail carries sufficient information for internalization. A point mutation in one of these 39 residues, K337 to R337, renders the receptor nonfunctional for endocytosis. Other residues, D335 and S338, contribute to the efficiency of internalization. When the sequence DAKSS is added onto a severely truncated receptor, endocytosis of the receptor is restored, showing that this sequence functions to mediate or to signal interaction with the endocytic machinery. Analysis of pheromone response and recovery in strains expressing mutant receptors suggests that receptor internalization is not important for response but contributes to recovery from pheromone.

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