Cytochemical Localization of Cyclic 3',5'-nucleotide Phosphodiesterase Activity in the Rat Olfactory Mucosa

Overview

Journal Histochem J

Specialty Biochemistry

Date 1993 May 1

PMID 8391521

Citations 2

Authors

N Asanuma

H Nomura

Affiliations

Soon will be listed here.

Abstract

Cyclic 3',5'-nucleotide phosphodiesterase activity was demonstrated cytochemically in the rat olfactory mucosa using cyclic AMP as substrate. Strong activity was observed on the plasma membrane of the cilia, dendritic knob and axon of olfactory cells; weak activity was apparent on the membrane of the dendritic shaft and cell body. This suggests that the cyclic AMP produced by odorant-sensitive adenylate cyclase in the dendritic terminal acts mainly in its original site and to a lesser extent in the dendritic shaft and cell body. The enzyme also hydrolysed cyclic GMP but the hydrolysis was not as great as in the case of cyclic AMP. Besides its presence in olfactory cells, enzymatic activity was also observed on the plasma membrane of basal cells and certain supporting cells with an astrocyte-like morphology.

Citing Articles

Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.

Kwon H, Koo J, Zufall F, Leinders-Zufall T, Margolis F PLoS One. 2009; 4(1):e4260.

PMID: 19165324 PMC: 2621343. DOI: 10.1371/journal.pone.0004260.

Cytochemical distribution of cyclic AMP-dependent 3',5'-nucleotide phosphodiesterase in the rat myocardium.

Okruhlicova L, Tribulova N, Eckly A, Lugnier C, Slezak J Histochem J. 1996; 28(3):165-72.

PMID: 8735283 DOI: 10.1007/BF02331440.

References

Borgers M . The cytochemical application of new potent inhibitors of alkaline phosphatases. J Histochem Cytochem. 1973; 21(9):812-24. DOI: 10.1177/21.9.812. View

Getchell T . Analysis of intracellular recordings from salamander olfactory epithelium. Brain Res. 1977; 123(2):275-86. DOI: 10.1016/0006-8993(77)90479-6. View

Boekhoff I, Tareilus E, Strotmann J, Breer H . Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants. EMBO J. 1990; 9(8):2453-8. PMC: 552272. DOI: 10.1002/j.1460-2075.1990.tb07422.x. View

Asanuma N, Nomura H . Cytochemical localization of adenylate cyclase activity in rat olfactory cells. Histochem J. 1991; 23(2):83-90. DOI: 10.1007/BF01047112. View

Sklar P, Anholt R, Snyder S . The odorant-sensitive adenylate cyclase of olfactory receptor cells. Differential stimulation by distinct classes of odorants. J Biol Chem. 1986; 261(33):15538-43. View

Kurahashi T . The response induced by intracellular cyclic AMP in isolated olfactory receptor cells of the newt. J Physiol. 1990; 430:355-71. PMC: 1181741. DOI: 10.1113/jphysiol.1990.sp018295. View

Rafols J, Getchell T . Morphological relations between the receptor neurons, sustentacular cells and Schwann cells in the olfactory mucosa of the salamander. Anat Rec. 1983; 206(1):87-101. DOI: 10.1002/ar.1092060111. View

Lowe G, Nakamura T, Gold G . Adenylate cyclase mediates olfactory transduction for a wide variety of odorants. Proc Natl Acad Sci U S A. 1989; 86(14):5641-5. PMC: 297680. DOI: 10.1073/pnas.86.14.5641. View

Ashcroft S, Randle P . Cyclic nucleotide phosphodiesterase activity in normal mouse pancreatic islets. FEBS Lett. 1972; 20(3):263-266. DOI: 10.1016/0014-5793(72)80082-6. View

10.

Nakamura T, Gold G . A cyclic nucleotide-gated conductance in olfactory receptor cilia. Nature. 1987; 325(6103):442-4. DOI: 10.1038/325442a0. View

11.

Breer H, Boekhoff I, Tareilus E . Rapid kinetics of second messenger formation in olfactory transduction. Nature. 1990; 345(6270):65-8. DOI: 10.1038/345065a0. View

12.

Frings S, Lindemann B . Current recording from sensory cilia of olfactory receptor cells in situ. I. The neuronal response to cyclic nucleotides. J Gen Physiol. 1991; 97(1):1-16. PMC: 2216467. DOI: 10.1085/jgp.97.1.1. View

13.

Vodyanoy V, Vodyanoy I . ATP and GTP are essential for olfactory response. Neurosci Lett. 1987; 73(3):253-8. DOI: 10.1016/0304-3940(87)90254-0. View

14.

Peytremann A, NICHOLSON W, LIDDLE G, Hardman J, SUTHERLAND E . Effects of methylxanthines on adenosine 3',5'-monophosphate and corticosterone in the rat adrenal. Endocrinology. 1973; 92(2):525-30. DOI: 10.1210/endo-92-2-525. View

15.

Firestein S, Zufall F, Shepherd G . Single odor-sensitive channels in olfactory receptor neurons are also gated by cyclic nucleotides. J Neurosci. 1991; 11(11):3565-72. PMC: 6575548. View

16.

Shirley S, Robinson C, Dickinson K, Aujla R, Dodd G . Olfactory adenylate cyclase of the rat. Stimulation by odorants and inhibition by Ca2+. Biochem J. 1986; 240(2):605-7. PMC: 1147457. DOI: 10.1042/bj2400605. View

17.

Cheung W . Cyclic 3',5'-nucleotide phosphodiesterase. Preparation of a partially inactive enzyme and its subsequent stimulation by snake venom. Biochim Biophys Acta. 1969; 191(2):303-15. DOI: 10.1016/0005-2744(69)90249-6. View

18.

Firestein S, Darrow B, Shepherd G . Activation of the sensory current in salamander olfactory receptor neurons depends on a G protein-mediated cAMP second messenger system. Neuron. 1991; 6(5):825-35. DOI: 10.1016/0896-6273(91)90178-3. View

19.

Hidaka H, Shibuya M, Asano T, Hara F . Cyclic nucleotide phosphodiesterase of human cerebrospinal fluid. J Neurochem. 1975; 25(1):49-53. DOI: 10.1111/j.1471-4159.1975.tb07692.x. View

20.

Cheung W . Cyclic 3',5'-nucleotide phosphodiesterase: pronounced stimulation by snake venom. Biochem Biophys Res Commun. 1967; 29(4):478-82. DOI: 10.1016/0006-291x(67)90508-6. View