Immunocytochemical Localization of Amiloride-sensitive Sodium Channels in the Lower Intestine of the Hen

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1993 Apr 1

PMID 8386985

Citations 1

Authors

P R Smith

A L Bradford

V Dantzer

D J Benos

E Skadhauge

Affiliations

Soon will be listed here.

Abstract

We have used polyclonal antibodies generated against purified bovine renal amiloride-sensitive Na+ channels to localize amiloride-sensitive Na+ channels within the lower intestine (colon and coprodeum) of the hen. These antibodies cross-reacted with two polypeptides exhibiting M(r)'s of 235 and 150 kDa on immunoblots of detergent-solubilized apical membrane fractions from both the colon and coprodeum. The apparent molecular masses of theses polypeptides are in agreement with the M(r)'s of 2 of the subunits of the renal high amiloride-affinity Na+ channel, namely the alpha and the beta (= amiloride binding) subunits. The cellular distribution of Na+ channels was determined by immunoperoxidase and indirect immunofluorescence cytochemical techniques. The apical (luminal) membrane and cytoplasm of villar principal cells in both colon and coprodeum exhibited immunoreactivity, whereas goblet cells were negative. Both principal and goblet cells of the crypts were also negative. We conclude that the amiloride-sensitive Na+ channels are localized to the principal cells of the intestinal villi and that these cells are responsible for intestinal Na+ absorption.

Citing Articles

Reconstitution of stretch-activated cation channels by expression of the alpha-subunit of the epithelial sodium channel cloned from osteoblasts.

Kizer N, Guo X, Hruska K Proc Natl Acad Sci U S A. 1997; 94(3):1013-8.

PMID: 9023374 PMC: 19631. DOI: 10.1073/pnas.94.3.1013.

References

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