» Articles » PMID: 8386823

Hepatitis B X Antigen and P53 Are Associated in Vitro and in Liver Tissues from Patients with Primary Hepatocellular Carcinoma

Overview
Journal Oncogene
Date 1993 May 1
PMID 8386823
Citations 82
Authors
Affiliations
Soon will be listed here.
Abstract

To test the hypothesis that hepatitis B x antigen (HBxAg) binds to the tumor-suppressor protein p53, immunoprecipitation was carried out with monoclonal anti-x or monoclonal anti-p53 using radiolabeled HBxAg and p53 made by in vitro translation. The results showed that anti-p53 specifically immunoprecipitates HBxAg only in the presence of p53 and that anti-x specifically immunoprecipitates p53 only in the presence of HBxAg. to determine whether HBxAg binds p53 in vivo, immunoprecipitation and Western blot analysis of liver samples from 10 hepatitis B virus (HBV)-infected patients with primary hepatocellular carcinoma (PHC) were carried out. A protein band at 53,000 daltons that specifically immunoprecipitated with a monoclonal anti-x was identified as p53 by Western blotting with a monoclonal anti-p53. Anti-p53 specifically immunoprecipitated bands of 28,000, 17,000 and 13,000 daltons, which were identified as HBxAg polypeptides by Western blotting with anti-HBx. These findings suggest that HBxAg binds to p53 and that this association is important to the development of PHC.

Citing Articles

Genetic Variants in p53 Pathway Genes Affect Survival of Patients with HBV-Related Hepatocellular Carcinoma.

Qin L, Qiu M, Tang J, Liu S, Lin Q, Huang Q J Hepatocell Carcinoma. 2024; 11:1541-1555.

PMID: 39156673 PMC: 11328861. DOI: 10.2147/JHC.S459792.


Transcriptomic identification of HBx-associated hub genes in hepatocellular carcinoma.

Ni Z, Lu J, Huang W, Khan H, Wu X, Huang D PeerJ. 2022; 9:e12697.

PMID: 35036167 PMC: 8710059. DOI: 10.7717/peerj.12697.


When Viruses Cross Developmental Pathways.

Trivedi P, Patel S, Bellavia D, Messina E, Palermo R, Ceccarelli S Front Cell Dev Biol. 2021; 9:691644.

PMID: 34422814 PMC: 8375270. DOI: 10.3389/fcell.2021.691644.


Short chain fatty acids delay the development of hepatocellular carcinoma in HBx transgenic mice.

McBrearty N, Arzumanyan A, Bichenkov E, Merali S, Merali C, Feitelson M Neoplasia. 2021; 23(5):529-538.

PMID: 33945993 PMC: 8111251. DOI: 10.1016/j.neo.2021.04.004.


Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells.

Zhang C, Yang H, Pan L, Zhao G, Zhang R, Zhang T Cancers (Basel). 2021; 13(5.

PMID: 33803301 PMC: 7967237. DOI: 10.3390/cancers13051181.