Hepatitis B X Antigen and P53 Are Associated in Vitro and in Liver Tissues from Patients with Primary Hepatocellular Carcinoma
Overview
Authors
Affiliations
To test the hypothesis that hepatitis B x antigen (HBxAg) binds to the tumor-suppressor protein p53, immunoprecipitation was carried out with monoclonal anti-x or monoclonal anti-p53 using radiolabeled HBxAg and p53 made by in vitro translation. The results showed that anti-p53 specifically immunoprecipitates HBxAg only in the presence of p53 and that anti-x specifically immunoprecipitates p53 only in the presence of HBxAg. to determine whether HBxAg binds p53 in vivo, immunoprecipitation and Western blot analysis of liver samples from 10 hepatitis B virus (HBV)-infected patients with primary hepatocellular carcinoma (PHC) were carried out. A protein band at 53,000 daltons that specifically immunoprecipitated with a monoclonal anti-x was identified as p53 by Western blotting with a monoclonal anti-p53. Anti-p53 specifically immunoprecipitated bands of 28,000, 17,000 and 13,000 daltons, which were identified as HBxAg polypeptides by Western blotting with anti-HBx. These findings suggest that HBxAg binds to p53 and that this association is important to the development of PHC.
Qin L, Qiu M, Tang J, Liu S, Lin Q, Huang Q J Hepatocell Carcinoma. 2024; 11:1541-1555.
PMID: 39156673 PMC: 11328861. DOI: 10.2147/JHC.S459792.
Transcriptomic identification of HBx-associated hub genes in hepatocellular carcinoma.
Ni Z, Lu J, Huang W, Khan H, Wu X, Huang D PeerJ. 2022; 9:e12697.
PMID: 35036167 PMC: 8710059. DOI: 10.7717/peerj.12697.
When Viruses Cross Developmental Pathways.
Trivedi P, Patel S, Bellavia D, Messina E, Palermo R, Ceccarelli S Front Cell Dev Biol. 2021; 9:691644.
PMID: 34422814 PMC: 8375270. DOI: 10.3389/fcell.2021.691644.
Short chain fatty acids delay the development of hepatocellular carcinoma in HBx transgenic mice.
McBrearty N, Arzumanyan A, Bichenkov E, Merali S, Merali C, Feitelson M Neoplasia. 2021; 23(5):529-538.
PMID: 33945993 PMC: 8111251. DOI: 10.1016/j.neo.2021.04.004.
Zhang C, Yang H, Pan L, Zhao G, Zhang R, Zhang T Cancers (Basel). 2021; 13(5.
PMID: 33803301 PMC: 7967237. DOI: 10.3390/cancers13051181.