Phospholipid Metabolism Under Muscarinic Cholinergic Stimulation Exhibits Brain Asymmetry

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1993 May 1

PMID 8386336

Citations 1

Authors

M F Pediconi

F J Barrantes

Affiliations

Soon will be listed here.

Abstract

In order to characterize some of the lateralized biochemical events promoted in brain upon massive neurotransmitter release, the labeling of lipids under specific stimulation of the muscarinic acetylcholine receptor (mAChR) has been studied in synaptosomes obtained from right and left cerebral cortex (RCC and LCC respectively). Synaptosomes were incubated with [32P]phosphate in the absence and in the presence of the cholinergic agonist carbamoylcholine and the muscarinic antagonist atropine. Binding of the agonist to the mAChR promoted an enhanced labeling of polyphosphoinositides, such effect being considerably more pronounced in the LCC than in the RCC. The differences observed could be due to a higher mAChR-elicited activity of phospholipase C in the RCC than in the LCC. The results show that mAChR stimulation activates the turnover of inositol lipids to a different extent in the two hemispheres, indicating either an uneven distribution of the receptor in brain and/or dissimilarities in the degree of coupling of the mAChR with its corresponding transmembrane signaling system in each hemicortex.

Citing Articles

Down-regulation of brain muscarinic cholinergic receptor promoted by diacylglycerols and phorbol ester.

Pediconi M, Barrantes F Neurochem Res. 1995; 20(10):1225-31.

PMID: 8746809 DOI: 10.1007/BF00995387.

References

Uma S, Ramakrishnan C . Studies on polyphosphoinositides in developing rat brain. J Neurochem. 1983; 40(4):914-6. DOI: 10.1111/j.1471-4159.1983.tb08073.x. View

Pediconi M, Rodriguez de Turco E . Free fatty acid content and release kinetics as manifestations of cerebral lateralization in mouse brain. J Neurochem. 1984; 43(1):1-7. DOI: 10.1111/j.1471-4159.1984.tb06671.x. View

Ginobili de Martinez M, Rodriguez de Turco E, Barrantes F . Endogenous asymmetry of rat brain lipids and dominance of the right cerebral hemisphere in free fatty acid response to electroconvulsive shock. Brain Res. 1985; 339(2):315-21. DOI: 10.1016/0006-8993(85)90097-6. View

Wastek G, Yamamura H . Biochemical characterization of the muscarinic cholinergic receptor in human brain: alterations in Huntington's disease. Mol Pharmacol. 1978; 14(5):768-80. View

Ginobili de Martinez M, Rodriguez de Turco E, Barrantes F . Asymmetry of diacylglycerol metabolism in rat cerebral hemispheres. J Neurochem. 1986; 46(5):1382-6. DOI: 10.1111/j.1471-4159.1986.tb01751.x. View

Bonner T . The molecular basis of muscarinic receptor diversity. Trends Neurosci. 1989; 12(4):148-51. DOI: 10.1016/0166-2236(89)90054-4. View

Shaikh N, Palmer F . Phosphoinositide kinases in chick brain and sciatic nerve, a developmental study. J Neurochem. 1977; 28(2):395-402. DOI: 10.1111/j.1471-4159.1977.tb07760.x. View

Rouser G, Fkeischer S, Yamamoto A . Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970; 5(5):494-6. DOI: 10.1007/BF02531316. View

Hauser G, EICHBERG J . Improved conditions for the preservation and extraction of polyphosphoinositides. Biochim Biophys Acta. 1973; 326(2):201-9. DOI: 10.1016/0005-2760(73)90246-4. View

10.

Ginobili de Martinez M, Barrantes F . Ca2+ and phospholipid-dependent protein kinase activity in rat cerebral hemispheres. Brain Res. 1988; 440(2):386-90. DOI: 10.1016/0006-8993(88)91013-x. View

11.

van Rooijen L, Seguin E, Agranoff B . Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranes. Biochem Biophys Res Commun. 1983; 112(3):919-26. DOI: 10.1016/0006-291x(83)91705-9. View

12.

De Graan P, Dekker L, de Wit M, Schrama L, Gispen W . Modulation of B-50 phosphorylation and polyphosphoinositide metabolism in synaptic plasma membranes by protein kinase C, phorbol diesters and ACTH. J Recept Res. 1988; 8(1-4):345-61. DOI: 10.3109/10799898809048997. View

13.

Fisher S, Figueiredo J, Bartus R . Differential stimulation of inositol phospholipid turnover in brain by analogs of oxotremorine. J Neurochem. 1984; 43(4):1171-9. DOI: 10.1111/j.1471-4159.1984.tb12858.x. View

14.

Aloyo V, Zwiers H, Gispen W . Phosphorylation of B-50 protein by calcium-activated, phospholipid-dependent protein kinase and B-50 protein kinase. J Neurochem. 1983; 41(3):649-53. DOI: 10.1111/j.1471-4159.1983.tb04790.x. View

15.

Pediconi M, Barrantes F . Brain asymmetry in phospholipid polar head group metabolism: parallel in vivo and in vitro studies. Neurochem Res. 1990; 15(1):25-32. DOI: 10.1007/BF00969180. View

16.

Richelson E . The molecular basis of neurotransmission at the muscarinic receptor. Biochem Pharmacol. 1981; 30(21):2887-91. DOI: 10.1016/0006-2952(81)90248-3. View

17.

Durell J, SODD M, Friedel R . Acetylcholine stimulation of the phosphodiesteratic cleavage of guinea pig brain phosphoinositides. Life Sci. 1968; 7(8):363-8. DOI: 10.1016/0024-3205(68)90034-9. View

18.

Abdel-Latif A . Calcium-mobilizing receptors, polyphosphoinositides, and the generation of second messengers. Pharmacol Rev. 1986; 38(3):227-72. View

19.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

20.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View