Characterization of Unrelated Strains of Staphylococcus Schleiferi by Using Ribosomal DNA Fingerprinting, DNA Restriction Patterns, and Plasmid Profiles

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1993 Apr 1

PMID 8385149

Citations 9

Authors

F Grattard

J Etienne

B Pozzetto

F Tardy

O G Gaudin

J Fleurette

Affiliations

Soon will be listed here.

Abstract

The molecular characteristics of 31 unrelated strains of Staphylococcus schleiferi isolated from 13 hospitals between 1973 and 1991 were determined by ribosomal DNA fingerprinting by using a digoxigenin-labeled DNA probe, genomic DNA restriction patterns, and plasmid profiles. Only six strains harbored one or two plasmids. DNA restriction analysis, which was carried out with five endonucleases (EcoRI, HindIII, PstI, PvuII, and ClaI), did not allow us to discriminate between isolates. Ribotyping with HindIII, ClaI, or EcoRI enzymes generated six, seven, and nine distinct patterns, respectively. With the combination ClaI-EcoRI, 13 ribotypes were obtained among the 31 strains, suggesting a relative heterogeneity within the species. Moreover, all strains shared two or three common bands, according to the endonuclease used, which were relatively specific for S. schleiferi in comparison with the ribosomal banding patterns described for other coagulase-negative staphylococci. These results illustrate that ribotyping can be used for the epidemiological investigation of S. schleiferi isolates and possibly for taxonomic analysis in this species.

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References

Skjold S, Quie P, Fries L, Barnham M, Cleary P . DNA fingerprinting of Streptococcus zooepidemicus (Lancefield group C) as an aid to epidemiological study. J Infect Dis. 1987; 155(6):1145-50. DOI: 10.1093/infdis/155.6.1145. View

Wilton J, Jung K, Vedin I, Aronsson B, Flock J . Comparative evaluation of a new molecular method for typing Staphylococcus epidermidis. Eur J Clin Microbiol Infect Dis. 1992; 11(6):515-21. DOI: 10.1007/BF01960806. View

Stull T, LiPuma J, Edlind T . A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988; 157(2):280-6. DOI: 10.1093/infdis/157.2.280. View

Etienne J, Brun Y, El Solh N, Delorme V, Mouren C, Bes M . Characterization of clinically significant isolates of Staphylococcus epidermidis from patients with endocarditis. J Clin Microbiol. 1988; 26(4):613-7. PMC: 266386. DOI: 10.1128/jcm.26.4.613-617.1988. View

Burnie J, Lee W . A comparison of DNA and immunoblot fingerprinting of the SII biotype of coagulase negative staphylococci. Epidemiol Infect. 1988; 101(2):203-12. PMC: 2249376. DOI: 10.1017/s095026880005411x. View

Renaud F, Freney J, Etienne J, Bes M, Brun Y, Barsotti O . Restriction endonuclease analysis of Staphylococcus epidermidis DNA may be a useful epidemiological marker. J Clin Microbiol. 1988; 26(9):1729-34. PMC: 266705. DOI: 10.1128/jcm.26.9.1729-1734.1988. View

Jean-Pierre H, Darbas H, Boyer G . Pathogenicity in two cases of Staphylococcus schleiferi, a recently described species. J Clin Microbiol. 1989; 27(9):2110-1. PMC: 267750. DOI: 10.1128/jcm.27.9.2110-2111.1989. View

Fleurette J, Bes M, Brun Y, Freney J, Forey F, COULET M . Clinical isolates of Staphylococcus lugdunensis and S. schleiferi: bacteriological characteristics and susceptibility to antimicrobial agents. Res Microbiol. 1989; 140(2):107-18. DOI: 10.1016/0923-2508(89)90044-2. View

Altwegg M, Mayer L . Bacterial molecular epidemiology based on a nonradioactive probe complementary to ribosomal RNA. Res Microbiol. 1989; 140(4-5):325-33. DOI: 10.1016/0923-2508(89)90024-7. View

10.

De Buyser M, Morvan A, Grimont F, El Solh N . Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns. J Gen Microbiol. 1989; 135(4):989-99. DOI: 10.1099/00221287-135-4-989. View

11.

Thomson-Carter F, Carter P, Pennington T . Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns. J Gen Microbiol. 1989; 135(7):2093-7. DOI: 10.1099/00221287-135-7-2093. View

12.

Bialkowska-Hobrzanska H, Jaskot D, Hammerberg O . Evaluation of restriction endonuclease fingerprinting of chromosomal DNA and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates. J Clin Microbiol. 1990; 28(2):269-75. PMC: 269589. DOI: 10.1128/jcm.28.2.269-275.1990. View

13.

Etienne J, Poitevin-Later F, Renaud F, Fleurette J . Plasmid profiles and genomic DNA restriction endonuclease patterns of 30 independent Staphylococcus lugdunensis strains. FEMS Microbiol Lett. 1990; 55(1-2):93-7. DOI: 10.1016/0378-1097(90)90175-p. View

14.

Brosius J, Ullrich A, Raker M, Gray A, Dull T, Gutell R . Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid. 1981; 6(1):112-8. DOI: 10.1016/0147-619x(81)90058-5. View

15.

Cooksey R, Baldwin J . Relatedness of tetracycline resistance plasmids among species of coagulase-negative staphylococci. Antimicrob Agents Chemother. 1985; 27(2):234-8. PMC: 176245. DOI: 10.1128/AAC.27.2.234. View

16.

Parisi J . Coagulase-negative staphylococci and the epidemiological typing of Staphylococcus epidermidis. Microbiol Rev. 1985; 49(2):126-39. PMC: 373026. DOI: 10.1128/mr.49.2.126-139.1985. View

17.

Bialkowska-Hobrzanska H, Harry V, Jaskot D, Hammerberg O . Typing of coagulase-negative staphylococci by Southern hybridization of chromosomal DNA fingerprints using a ribosomal RNA probe. Eur J Clin Microbiol Infect Dis. 1990; 9(8):588-94. DOI: 10.1007/BF01967213. View

18.

HEBERT G . Hemolysins and other characteristics that help differentiate and biotype Staphylococcus lugdunensis and Staphylococcus schleiferi. J Clin Microbiol. 1990; 28(11):2425-31. PMC: 268200. DOI: 10.1128/jcm.28.11.2425-2431.1990. View

19.

Graves L, Swaminathan B, Reeves M, Wenger J . Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labeled DNA probe. Eur J Epidemiol. 1991; 7(1):77-82. DOI: 10.1007/BF00221345. View

20.

Izard N, Hachler H, Grehn M, Kayser F . Ribotyping of coagulase-negative staphylococci with special emphasis on intraspecific typing of Staphylococcus epidermidis. J Clin Microbiol. 1992; 30(4):817-23. PMC: 265168. DOI: 10.1128/jcm.30.4.817-823.1992. View