Light-induced Carotenogenesis in Myxococcus Xanthus: Genetic Analysis of the CarR Region

Overview

Journal Mol Microbiol

Specialties Microbiology
Molecular Biology

Date 1993 Feb 1

PMID 8384685

Citations 19

Authors

D A Hodgson

Affiliations

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Abstract

Carotenogenesis is light-inducible in the non-photosynthetic, Gram-negative, bacterium Myxococcus xanthus. We report the characterization of the carR region which controls this phenomenon. Insertion of transposon Tn5 close to the carR region caused a dominant, carotenoid-constitutive mutation because of the presence of a constitutive, outward-reading promoter in the IS50L component of Tn5. In wild-type cells, a powerful, tightly-regulated, light-inducible promoter directs the transcription of two genetic functions. One of these functions is to activate transcription of the genetically unlinked carB gene, which is involved in carotenoid synthesis. The second function (carR) regulates the light-inducible promoter. We also report the mapping of two carotenoid constitutive mutations to the previously characterized carA locus.

Citing Articles

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Structure-function dissection of Myxococcus xanthus CarD N-terminal domain, a defining member of the CarD_CdnL_TRCF family of RNA polymerase interacting proteins.

Bernal-Bernal D, Gallego-Garcia A, Garcia-Martinez G, Garcia-Heras F, Jimenez M, Padmanabhan S PLoS One. 2015; 10(3):e0121322.

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CarF mediates signaling by singlet oxygen, generated via photoexcited protoporphyrin IX, in Myxococcus xanthus light-induced carotenogenesis.

Galbis-Martinez M, Padmanabhan S, Murillo F, Elias-Arnanz M J Bacteriol. 2012; 194(6):1427-36.

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