A Beta-adrenergic Receptor Kinase-like Enzyme is Involved in Olfactory Signal Termination
Overview
Affiliations
We have previously shown that second-messenger-dependent kinases (cAMP-dependent kinase, protein kinase C) in the olfactory system are essential in terminating second-messenger signaling in response to odorants. We now document that subtype 2 of the beta-adrenergic receptor kinase (beta ARK) is also involved in this process. By using subtype-specific antibodies to beta ARK-1 and beta ARK-2, we show that beta ARK-2 is preferentially expressed in the olfactory epithelium in contrast to findings in most other tissues. Heparin, an inhibitor of beta ARK, as well as anti-beta ARK-2 antibodies, (i) completely prevents the rapid decline of second-messenger signals (desensitization) that follows odorant stimulation and (ii) strongly inhibits odorant-induced phosphorylation of olfactory ciliary proteins. In contrast, beta ARK-1 antibodies are without effect. Inhibitors of protein kinase A and protein kinase C also block odorant-induced desensitization and phosphorylation. These data suggest that a sequential interplay of second-messenger-dependent and receptor-specific kinases is functionally involved in olfactory desensitization.
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Jiang H, Galtes D, Wang J, Rockman H Am J Physiol Cell Physiol. 2022; 323(3):C731-C748.
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Genovese F, Reisert J, Kefalov V Front Cell Neurosci. 2021; 15:761416.
PMID: 34690705 PMC: 8531253. DOI: 10.3389/fncel.2021.761416.
Zimering M, Delic V, Citron B Mol Neurobiol. 2021; 58(9):4365-4375.
PMID: 34013450 PMC: 8487420. DOI: 10.1007/s12035-021-02428-4.
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Topalidou I, Cooper K, Pereira L, Ailion M PLoS Genet. 2017; 13(10):e1007032.
PMID: 28968387 PMC: 5638609. DOI: 10.1371/journal.pgen.1007032.