Mechanisms for Sister Chromatid Exchanges and Their Relation to the Production of Chromosomal Aberrations

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1977 Feb 3

PMID 837800

Citations 22

Authors

H Kato

Affiliations

Soon will be listed here.

Abstract

By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out of four DNA strands, BrdU-substitution had occurred either in one or three strands. The FL-induced SCE frequency did not differ greatly between these two types of chromosomes. However, when they were submitted to caffeine treatment, a drastic increase in the frequency was detected in the trifilarly-substituted chromosomes while a significant decrease occurred in the unifilarly-substituted chromosomes. Based on these results, a working hypothesis was developed that the SCE can arise by at least two different mechanisms, one operating at replicating points probably utilizing the machinery of DNA replication, and the other acting only in the post-replicational DNA portion, probably in a similar fashion as assumed in a general model of crossing over in the eukaryote. These dual mechansims may account for the discrepancy encountered in the explanations of the induction of SCEs by various exogenous agents as well as spontaneous SCEs. The present study also showed that some, but clearly not all, of chromatid deletions are the outcome of the failure to complete SCEs arising through these mechanisms.

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References

Heddle J, Bodycote D . On the formation of chromosomal aberrations. Mutat Res. 1970; 9(1):117-26. DOI: 10.1016/0027-5107(70)90075-8. View

Gatti M, Pimpinelli S, Olivieri G . The frequency and distribution of isolabelling in Chinese hamster chromosomes after exposure to x-rays. Mutat Res. 1974; 23(2):229-38. DOI: 10.1016/0027-5107(74)90143-2. View

Rommelaere J, Susskind M, ERRERA M . Chromosome and chromatid exchanges in Chinese hamster cells. Chromosoma. 1973; 41(3):243-57. DOI: 10.1007/BF00344019. View

Marin G, Prescott D . THE FREQUENCY OF SISTER CHROMATID EXCHANGES FOLLOWING EXPOSURE TO VARYING DOSES OF H3-THYMIDINE OR X-RAYS. J Cell Biol. 1964; 21:159-67. PMC: 2106437. DOI: 10.1083/jcb.21.2.159. View

Fujiwara Y . Characteristics of DNA synthesis following ultraviolet light irradiation in mouse L cells. Postreplication repair. Exp Cell Res. 1972; 75(2):485-9. DOI: 10.1016/0014-4827(72)90456-9. View

Whitehouse H . A THEORY OF CROSSING-OVER BY MEANS OF HYBRID DEOXYRIBONUCLEIC ACID. Nature. 1963; 199:1034-40. DOI: 10.1038/1991034a0. View

LATT S . Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci U S A. 1973; 70(12):3395-9. PMC: 427244. DOI: 10.1073/pnas.70.12.3395. View

Amaldi F, Carnevali F, Leoni L, Mariotti D . Replicon origins in Chinese hamsters cell DNA. I. Labeling procedure and preliminary observations. Exp Cell Res. 1972; 74(2):367-74. DOI: 10.1016/0014-4827(72)90389-8. View

Lehmann A . Postreplication repair of DNA in ultraviolet-irradiated mammalian cells. J Mol Biol. 1972; 66(3):319-37. DOI: 10.1016/0022-2836(72)90418-4. View

10.

Wolff S, Perry P . Differential Giemsa staining of sister chromatids and the study of chromatid exchanges without autoradiography. Chromosoma. 1974; 48(4):341-53. DOI: 10.1007/BF00290991. View

11.

Korenberg J, Freedlender E . Giemsa technique for the detection of sister chromatid exchanges. Chromosoma. 1974; 48(4):355-60. DOI: 10.1007/BF00290992. View

12.

Kato H . Induction of sister chromatid exchanges by UV light and its inhibition by caffeine. Exp Cell Res. 1973; 82(2):383-90. DOI: 10.1016/0014-4827(73)90356-x. View

13.

Wolff S, Bodycote J, Painter R . Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: the necessity for cells to pass through S. Mutat Res. 1974; 25(1):73-81. DOI: 10.1016/0027-5107(74)90220-6. View

14.

Ikushima T, Wolff S . Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes. Exp Cell Res. 1974; 87(1):15-9. DOI: 10.1016/0014-4827(74)90521-7. View

15.

Smets L, Cornelis J . Repairable and irrepairable damage in 5-bromouracil-substituted DNA exposed to ultra-violet radiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1971; 19(5):445-57. DOI: 10.1080/09553007114550581. View

16.

Lindahl T, Andersson A . Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid. Biochemistry. 1972; 11(19):3618-23. DOI: 10.1021/bi00769a019. View

17.

Sobell H . Symmetry in protein-nucleic acid interaction and its genetic implications. Adv Genet. 1973; 17:411-90. DOI: 10.1016/s0065-2660(08)60175-3. View

18.

Kato H . Induction of sister chromatid exchanges by chemical mutagens and its possible relevance to DNA repair. Exp Cell Res. 1974; 85(2):239-47. DOI: 10.1016/0014-4827(74)90123-2. View

19.

Wolff S, Bodycote J . The induction of chromatid deletions in accord with the breakage-and-reunion hypothesis. Mutat Res. 1975; 29(1):85-91. DOI: 10.1016/0027-5107(75)90023-8. View

20.

Taylor J . Sister Chromatid Exchanges in Tritium-Labeled Chromosomes. Genetics. 1958; 43(3):515-29. PMC: 1209899. DOI: 10.1093/genetics/43.3.515. View