Schizosaccharomyces Pombe Ypt5: a Homologue of the Rab5 Endosome Fusion Regulator

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 1993 Jun 1

PMID 8374169

Citations 13

Authors

J Armstrong

M W Craighead

R Watson

S Ponnambalam

S Bowden

Affiliations

Soon will be listed here.

Abstract

The ypt/rab proteins are a family of small GTP-binding proteins thought to be required for different stages of membrane traffic. From the fission yeast Schizosaccharomyces pombe we have isolated and characterized ypt5, a gene encoding a homologue of rab5, a mammalian protein apparently involved in regulating fusion of early endosomes. Recombinant ypt5 protein bound GTP. The ypt5 gene was found to be essential for viability on minimal media, but ypt5-disrupted cells grew slowly on some rich media and accumulated a population of small vesicles not observed in wild-type cells. Canine rab5 cDNA could replace the ypt5 gene in S. pombe and restore normal growth and viability. Ypt5 protein expressed in mammalian cells colocalized with the transferrin receptor to early endosomes. Thus, molecular aspects of the early endocytic pathway may be conserved between mammalian cells and S. pombe and hence may be amenable to genetic analysis.

Citing Articles

Two Rab5 Homologs Are Essential for the Development and Pathogenicity of the Rice Blast Fungus .

Yang C, Dang X, Zheng H, Chen X, Lin X, Zhang D Front Plant Sci. 2017; 8:620.

PMID: 28529514 PMC: 5418346. DOI: 10.3389/fpls.2017.00620.

Essential role for Schizosaccharomyces pombe pik1 in septation.

Park J, Steinbach S, Desautels M, Hemmingsen S PLoS One. 2009; 4(7):e6179.

PMID: 19587793 PMC: 2704394. DOI: 10.1371/journal.pone.0006179.

Endocytosis in the shiitake mushroom Lentinula edodes and involvement of GTPase LeRAB7.

Lee M, Szeto C, Ng T, Kwan H Eukaryot Cell. 2007; 6(12):2406-18.

PMID: 17921351 PMC: 2168259. DOI: 10.1128/EC.00222-07.

A novel phosphatidylinositol(3,4,5)P3 pathway in fission yeast.

Mitra P, Zhang Y, Rameh L, Ivshina M, McCollum D, Nunnari J J Cell Biol. 2004; 166(2):205-11.

PMID: 15249580 PMC: 2172303. DOI: 10.1083/jcb.200404150.

Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast.

Kita A, Sugiura R, Shoji H, He Y, Deng L, Lu Y Mol Biol Cell. 2004; 15(6):2920-31.

PMID: 15047861 PMC: 420114. DOI: 10.1091/mbc.e03-09-0659.

References

Chavrier P, Gorvel J, Stelzer E, Simons K, Gruenberg J, Zerial M . Hypervariable C-terminal domain of rab proteins acts as a targeting signal. Nature. 1991; 353(6346):769-72. DOI: 10.1038/353769a0. View

Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M . Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc Natl Acad Sci U S A. 1981; 78(7):4515-9. PMC: 319822. DOI: 10.1073/pnas.78.7.4515. View

Davey J . Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone. EMBO J. 1992; 11(3):951-60. PMC: 556536. DOI: 10.1002/j.1460-2075.1992.tb05134.x. View

Chavrier P, Simons K, Zerial M . The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach. Gene. 1992; 112(2):261-4. DOI: 10.1016/0378-1119(92)90387-5. View

Elferink L, Anzai K, Scheller R . rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain. J Biol Chem. 1992; 267(9):5768-75. View

Pidoux A, Armstrong J . Analysis of the BiP gene and identification of an ER retention signal in Schizosaccharomyces pombe. EMBO J. 1992; 11(4):1583-91. PMC: 556607. DOI: 10.1002/j.1460-2075.1992.tb05203.x. View

Fawell E, Bowden S, Armstrong J . A homologue of the ras-related CDC42 gene from Schizosaccharomyces pombe. Gene. 1992; 114(1):153-4. DOI: 10.1016/0378-1119(92)90724-4. View

Newman C, Giannakouros T, Hancock J, Fawell E, Armstrong J, Magee A . Post-translational processing of Schizosaccharomyces pombe YPT proteins. J Biol Chem. 1992; 267(16):11329-36. View

Bucci C, Parton R, Mather I, Stunnenberg H, Simons K, Hoflack B . The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell. 1992; 70(5):715-28. DOI: 10.1016/0092-8674(92)90306-w. View

10.

van der Sluijs P, Hull M, Webster P, MALE P, Goud B, Mellman I . The small GTP-binding protein rab4 controls an early sorting event on the endocytic pathway. Cell. 1992; 70(5):729-40. DOI: 10.1016/0092-8674(92)90307-x. View

11.

Raths S, Rohrer J, Crausaz F, Riezman H . end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol. 1993; 120(1):55-65. PMC: 2119492. DOI: 10.1083/jcb.120.1.55. View

12.

Wichmann H, Hengst L, Gallwitz D . Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p). Cell. 1992; 71(7):1131-42. DOI: 10.1016/s0092-8674(05)80062-5. View

13.

Tabor S, Richardson C . DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987; 84(14):4767-71. PMC: 305186. DOI: 10.1073/pnas.84.14.4767. View

14.

Mertins P, Gallwitz D . Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element. EMBO J. 1987; 6(6):1757-63. PMC: 553552. DOI: 10.1002/j.1460-2075.1987.tb02428.x. View

15.

Touchot N, Chardin P, Tavitian A . Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library. Proc Natl Acad Sci U S A. 1987; 84(23):8210-4. PMC: 299511. DOI: 10.1073/pnas.84.23.8210. View

16.

Segev N, Mulholland J, Botstein D . The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell. 1988; 52(6):915-24. DOI: 10.1016/0092-8674(88)90433-3. View

17.

Haubruck H, Disela C, Wagner P, Gallwitz D . The ras-related ypt protein is an ubiquitous eukaryotic protein: isolation and sequence analysis of mouse cDNA clones highly homologous to the yeast YPT1 gene. EMBO J. 1987; 6(13):4049-53. PMC: 553887. DOI: 10.1002/j.1460-2075.1987.tb02750.x. View

18.

Smith D, Svoboda A . Golgi apparatus in normal cells and protoplasts of Schizosaccharomyces pombe. Microbios. 1972; 5(19):177-82. View

19.

Nilsson T, Jackson M, Peterson P . Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum. Cell. 1989; 58(4):707-18. DOI: 10.1016/0092-8674(89)90105-0. View

20.

Riezman H . Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis. Cell. 1985; 40(4):1001-9. DOI: 10.1016/0092-8674(85)90360-5. View