In Vivo Flow Visualization of Induced Saccular Cerebral Aneurysms in Rats

Overview

Journal Acta Neurochir (Wien)

Specialty Neurosurgery

Date 1993 Jan 1

PMID 8372716

Citations 4

Authors

H Nakatani

N Hashimoto

H Kikuchi

S Yamaguchi

H Niimi

Affiliations

Soon will be listed here.

Abstract

A surgical procedure to expose the arterial bifurcation at the base of the rat brain was developed without sacrificing the animal. Using this technique, visualization of flow in and around the induced cerebral aneurysm was achieved by detecting and following fluorescent particles in the blood stream. Cerebral aneurysms were produced by ligating one common carotid artery, inducing experimental hypertension and feeding them with beta-aminopropionitrile. Flow studies of the arterial bifurcation with an early aneurysmal formation showed that there were spiral flows proximal and distal to the bifurcation. This was the first direct visualization of the actual flow in and around cerebral aneurysms in a vital state. This technology can add further information on the development, growth and rupture of cerebral aneurysms.

Citing Articles

Imaging of intracranial aneurysms in animals: a systematic review of modalities.

Cayron A, Morel S, Allemann E, Bijlenga P, Kwak B Neurosurg Rev. 2023; 46(1):56.

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Endogenous animal models of intracranial aneurysm development: a review.

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Preclinical Intracranial Aneurysm Models: A Systematic Review.

Strange F, Gruter B, Fandino J, Marbacher S Brain Sci. 2020; 10(3).

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Effect of flow diverter porosity on intraaneurysmal blood flow.

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References

Hashimoto N, Kim C, Kikuchi H, Kojima M, Kang Y, Hazama F . Experimental induction of cerebral aneurysms in monkeys. J Neurosurg. 1987; 67(6):903-5. DOI: 10.3171/jns.1987.67.6.0903. View

Karino T, Motomiya M . Flow visualization in isolated transparent natural blood vessels. Biorheology. 1983; 20(2):119-27. DOI: 10.3233/bir-1983-20202. View

Steiger H . Pathophysiology of development and rupture of cerebral aneurysms. Acta Neurochir Suppl (Wien). 1990; 48:1-57. View

Hale S, Vivaldi M, Kloner R . Fluorescent microspheres: a new tool for visualization of ischemic myocardium in rats. Am J Physiol. 1986; 251(4 Pt 2):H863-8. DOI: 10.1152/ajpheart.1986.251.4.H863. View

Hazama F, Kataoka H, Yamada E, Kayembe K, Hashimoto N, Kojima M . Early changes of experimentally induced cerebral aneurysms in rats. Light-microscopic study. Am J Pathol. 1986; 124(3):399-404. PMC: 1888351. View

McDonagh P, Niven A, Roberts D . Direct visualization of the coronary microcirculation for pharmacologic and physiologic studies. Microvasc Res. 1984; 28(2):180-96. DOI: 10.1016/0026-2862(84)90016-5. View

Kim C, Kikuchi H, Hashimoto N, Kojima M, Kang Y, Hazama F . Involvement of internal elastic lamina in development of induced cerebral aneurysms in rats. Stroke. 1988; 19(4):507-11. DOI: 10.1161/01.str.19.4.507. View

Hashimoto N, Handa H, Nagata I, Hazama F . Animal model of cerebral aneurysms: pathology and pathogenesis of induced cerebral aneurysms in rats. Neurol Res. 1984; 6(1-2):33-40. DOI: 10.1080/01616412.1984.11739661. View

Nakatani H, Hashimoto N, Kang Y, Yamazoe N, Kikuchi H, Yamaguchi S . Cerebral blood flow patterns at major vessel bifurcations and aneurysms in rats. J Neurosurg. 1991; 74(2):258-62. DOI: 10.3171/jns.1991.74.2.0258. View

10.

Nuttall A . Techniques for the observation and measurement of red blood cell velocity in vessels of the guinea pig cochlea. Hear Res. 1987; 27(2):111-9. DOI: 10.1016/0378-5955(87)90012-8. View

11.

Steiger H, Poll A, Liepsch D, Reulen H . Basic flow structure in saccular aneurysms: a flow visualization study. Heart Vessels. 1987; 3(2):55-65. DOI: 10.1007/BF02058520. View

12.

Fukushima T, Homma T, Azuma T, Harakawa K . Characteristics of secondary flow in steady and pulsatile flows through a symmetrical bifurcation. Biorheology. 1987; 24(1):3-12. DOI: 10.3233/bir-1987-24102. View

13.

Niimi H, Kawano Y, Sugiyama I . Structure of blood flow through a curved vessel with an aneurysm. Biorheology. 1984; 21(4):603-15. DOI: 10.3233/bir-1984-21418. View

14.

Kojima M, Handa H, Hashimoto N, Kim C, Hazama F . Early changes of experimentally induced cerebral aneurysms in rats: scanning electron microscopic study. Stroke. 1986; 17(5):835-41. DOI: 10.1161/01.str.17.5.835. View

15.

Stehbens W . Flow in glass models of arterial bifurcations and berry aneurysms at low Reynolds numbers. Q J Exp Physiol Cogn Med Sci. 1975; 60(3):181-92. DOI: 10.1113/expphysiol.1975.sp002310. View

16.

Tangelder G, Slaaf D, Muijtjens A, Arts T, oude Egbrink M, Reneman R . Velocity profiles of blood platelets and red blood cells flowing in arterioles of the rabbit mesentery. Circ Res. 1986; 59(5):505-14. DOI: 10.1161/01.res.59.5.505. View