The Fission Yeast Prp4+ Gene Involved in Pre-mRNA Splicing Codes for a Predicted Serine/threonine Kinase and is Essential for Growth

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1993 Aug 25

PMID 8371982

Citations 25

Authors

S K Alahari

H Schmidt

N F Kaufer

Affiliations

Soon will be listed here.

Abstract

Only four prp (pre-mRNA processing) genes of the fission yeast Schizosaccharomyces pombe have been reported. We exploited yeast genetics and identified and isolated the prp4 gene. Sequence analysis revealed that the splicing factor encoded by this gene contains the signature sequences that define the serine/threonine protein kinase family. This is the first kinase gene identified whose product is involved in pre-mRNA splicing. The prp4 gene contains one intron in the kinase domain. Gene replacement studies provided evidence that this gene is essential for growth and is located on chromosome III.

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