Cloning and Expression of Hormoconis Resinae Glucoamylase P CDNA in Saccharomyces Cerevisiae

Overview

Journal Curr Genet

Specialty Genetics

Date 1993 Jul 1

PMID 8358830

Citations 4

Authors

A E Vainio

H T Torkkeli

T Tuusa

S A Aho

B R Fagerstrom

M P Korhola

Affiliations

Soon will be listed here.

Abstract

A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37-48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.

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