Peroxisome Biogenesis in the Yeast Hansenula Polymorpha is Controlled by a Complex Set of Interacting Gene Products

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Aug 15

PMID 8356040

Citations 10

Authors

V I Titorenko

H R Waterham

J M Cregg

W Harder

M Veenhuis

Affiliations

Soon will be listed here.

Abstract

We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-); (ii) presence of small peroxisomes in conjunction with a major fraction of peroxisomal matrix proteins in the cytosol (Pim-); and (iii) presence of peroxisomes with aberrant crystalline matrix substructure (Pss-). Extensive complementation analysis showed many cases of noncomplementation--that is, diploids that contained both wild-type and mutant alleles of two different PER genes were unable to grow on methanol and showed peroxisomal defects. The observed cases of unlinked noncomplementation appeared to be gene and allele specific and were predominantly observed at lower temperatures (cold sensitive). The genetic results obtained were used to formulate a model of PER gene product interactions. In this model, five PER gene products are key or core components of the complex. Other PER gene products appear to play a more peripheral role.

Citing Articles

Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase.

Ozimek P, van Dijk R, Latchev K, Gancedo C, Wang D, van der Klei I Mol Biol Cell. 2003; 14(2):786-97.

PMID: 12589070 PMC: 150008. DOI: 10.1091/mbc.e02-07-0417.

The ubiquitin-conjugating enzyme Pex4p of Hansenula polymorpha is required for efficient functioning of the PTS1 import machinery.

van der Klei I, Hilbrands R, Kiel J, Rasmussen S, Cregg J, Veenhuis M EMBO J. 1998; 17(13):3608-18.

PMID: 9649431 PMC: 1170697. DOI: 10.1093/emboj/17.13.3608.

Noncomplementation of radiation-induced chromosome aberrations in ataxia-telangiectasia/ataxia-telangiectasia-variant heterodikaryons.

Stumm M, Sperling K, Wegner R Am J Hum Genet. 1997; 60(5):1246-51.

PMID: 9150175 PMC: 1712422.

The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis.

Komori M, Rasmussen S, Kiel J, Baerends R, Cregg J, van der Klei I EMBO J. 1997; 16(1):44-53.

PMID: 9009266 PMC: 1169612. DOI: 10.1093/emboj/16.1.44.

Characterization of peroxisome-deficient mutants of Hansenula polymorpha.

Tan X, Titorenko V, van der Klei I, Sulter G, Haima P, Waterham H Curr Genet. 1995; 28(3):248-57.

PMID: 8529271 DOI: 10.1007/BF00309784.

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