Growth and Invasion of Human Melanomas in Human Skin Grafted to Immunodeficient Mice

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1993 Aug 1

PMID 8342600

Citations 48

Authors

I Juhasz

S M Albelda

D E Elder

G F Murphy

K Adachi

D Herlyn

M Herlyn

Affiliations

Soon will be listed here.

Abstract

An orthotopic model of human melanoma was developed in which malignant cells were injected into human skin grafted to nude and SCID mice. Melanoma cells proliferated and invaded the human skin grafts with characteristic patterns. Three of six melanomas grew as multiple nodules and infiltered the grafts without major architectural changes in the dermis, whereas the others invaded the dermis along collagen fibers with prominent endothelial vessels. By contrast, melanoma cells inoculated into mouse skin grew as diffusely expanding nodules that did not invade the murine dermis. In human skin grafts, human melanoma cells were angiogenic for human blood vessels, and murine vessels were only found at the periphery of grafts. Tumor cells invaded the human vessels, and four out of seven cell lines metastasized to lungs, suggesting that this model is useful to determine in vivo the interactions between normal and malignant human cells.

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References

GIOVANELLA B, Yim S, STEHLIN J, Williams Jr L . Development of invasive tumors in the "nude" mouse after injection of cultured human melanoma cells. J Natl Cancer Inst. 1972; 48(5):1531-3. View

Balaban G, Herlyn M, Guerry 4th D, Bartolo R, KOPROWSKI H, Clark W . Cytogenetics of human malignant melanoma and premalignant lesions. Cancer Genet Cytogenet. 1984; 11(4):429-39. DOI: 10.1016/0165-4608(84)90024-4. View

Kozlowski J, Fidler I, Campbell D, Xu Z, KAIGHN M, Hart I . Metastatic behavior of human tumor cell lines grown in the nude mouse. Cancer Res. 1984; 44(8):3522-9. View

Cunha G, Vanderslice K . Identification in histological sections of species origin of cells from mouse, rat and human. Stain Technol. 1984; 59(1):7-12. DOI: 10.3109/10520298409113823. View

Atkinson B, Ernst C, Ghrist B, Ross A, Clark W, Herlyn M . Monoclonal antibody to a highly glycosylated protein reacts in fixed tissue with melanoma and other tumors. Hybridoma. 1985; 4(3):243-55. DOI: 10.1089/hyb.1985.4.243. View

Gown A, Vogel A, Hoak D, Gough F, McNutt M . Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol. 1986; 123(2):195-203. PMC: 1888307. View

Fidler I . Rationale and methods for the use of nude mice to study the biology and therapy of human cancer metastasis. Cancer Metastasis Rev. 1986; 5(1):29-49. DOI: 10.1007/BF00049529. View

Westermark B, Johnsson A, Paulsson Y, Betsholtz C, Heldin C, Herlyn M . Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor (PDGF) and produce a PDGF-like growth factor. Proc Natl Acad Sci U S A. 1986; 83(19):7197-200. PMC: 386682. DOI: 10.1073/pnas.83.19.7197. View

Folkman J, Klagsbrun M . Angiogenic factors. Science. 1987; 235(4787):442-7. DOI: 10.1126/science.2432664. View

10.

Naito S, Von Eschenbach A, Fidler I . Different growth pattern and biologic behavior of human renal cell carcinoma implanted into different organs of nude mice. J Natl Cancer Inst. 1987; 78(2):377-85. View

11.

Herlyn M, Rodeck U, Mancianti M, Cardillo F, Lang A, Ross A . Expression of melanoma-associated antigens in rapidly dividing human melanocytes in culture. Cancer Res. 1987; 47(12):3057-61. View

12.

McLemore T, Liu M, Blacker P, Gregg M, Alley M, ABBOTT B . Novel intrapulmonary model for orthotopic propagation of human lung cancers in athymic nude mice. Cancer Res. 1987; 47(19):5132-40. View

13.

Wilson E, Gartner M, Campbell J, DOWDLE E . Metastasis of a human melanoma cell line in the nude mouse. Int J Cancer. 1988; 41(1):83-6. DOI: 10.1002/ijc.2910410116. View

14.

Dore J, Bailly M, Bertrand S . Metastases of human tumors in experimental animals. Anticancer Res. 1987; 7(5B):997-1003. View

15.

Fodstad O, Aamdal S, McMenamin M, Nesland J, Pihl A . A new experimental metastasis model in athymic nude mice, the human malignant melanoma LOX. Int J Cancer. 1988; 41(3):442-9. DOI: 10.1002/ijc.2910410322. View

16.

Fodstad O, Kjonniksen I, Aamdal S, Nesland J, Boyd M, Pihl A . Extrapulmonary, tissue-specific metastasis formation in nude mice injected with FEMX-I human melanoma cells. Cancer Res. 1988; 48(15):4382-8. View

17.

Morikawa K, Walker S, Nakajima M, Pathak S, Jessup J, Fidler I . Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice. Cancer Res. 1988; 48(23):6863-71. View

18.

Ormerod E, Hart I . Different growth responses to agents which elevate cAMP in human melanoma cell lines of high and low experimental metastatic capacity. Clin Exp Metastasis. 1989; 7(1):85-95. DOI: 10.1007/BF02057183. View

19.

Thiede K, Momburg F, Zangemeister U, Schlag P, Schirrmacher V . Growth and metastasis of human tumors in nude mice following tumor-cell inoculation into a vascularized polyurethane sponge matrix. Int J Cancer. 1988; 42(6):939-45. DOI: 10.1002/ijc.2910420625. View

20.

Halaban R, Kwon B, Ghosh S, Delli Bovi P, Baird A . bFGF as an autocrine growth factor for human melanomas. Oncogene Res. 1988; 3(2):177-86. View