Cloning and Characterization of the Gamma-glutamyl Phosphate Reductase Gene of Campylobacter Jejuni

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1993 Jul 1

PMID 8341262

Citations 8

Authors

H Louie

V L Chan

Affiliations

Soon will be listed here.

Abstract

The gamma-glutamyl phosphate reductase gene, proA, of Campylobacter jejuni was isolated from a recombinant pBR322 clone. A HindIII fragment of the insert containing the gene was subcloned into pUC19 and sequenced in both orientations. The deduced amino acid sequence of gamma-glutamyl phosphate reductase (EC 1.2.1.41) of C. jejuni exhibits 36.4% identity to that of Escherichia coli and 36.0% identity to Serratia marcescens. Two highly conserved regions in the amino acid sequence were identified from the alignment of the three available gamma-glutamyl phosphate reductase gene sequences. The gene was expressed from its own promoter and the transcription start site was mapped. The proline biosynthetic genes of C. jejuni are not located tandemly and thus differ in this respect from those of E. coli and S. marcescens, where gamma-glutamyl phosphate reductase and gamma-glutamyl kinase (proB) are located in a single operon.

Citing Articles

Campylobacter upsaliensis: waiting in the wings.

Bourke B, Chan V, Sherman P Clin Microbiol Rev. 1998; 11(3):440-9.

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Identification of Campylobacter jejuni promoter sequences.

Wosten M, Boeve M, Koot M, van Nuenen A, van der Zeijst B J Bacteriol. 1998; 180(3):594-9.

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Mutations in the Corynebacterium glutamicum proline biosynthetic pathway: a natural bypass of th proA step.

Ankri S, Serebrijski I, Reyes O, Leblon G J Bacteriol. 1996; 178(15):4412-9.

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Corrected gene assignments of Escherichia coli pro- mutations.

Serebrijski I, Reyes O, Leblon G J Bacteriol. 1995; 177(24):7261-4.

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Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants.

Serebrijski I, Wojcik F, Reyes O, Leblon G J Bacteriol. 1995; 177(24):7255-60.

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