Alpha 2.0
Login Register
» Articles » PMID: 833931

Polypeptides of Respiratory Syncytial Virus

Overview
Journal J Virol
Publisher American Society for Microbiology
Date 1977 Jan 1
PMID 833931
Citations 26
Authors
S Levine
Affiliations
Soon will be listed here.
Abstract

Radiolabeled respiratory syncytial virus was purified from medium that had been harvested from infected HeLa cell monolayers before it contained much cellular debris. After isopycnic centrifugation in linear gradients prepared with sucrose dissolved in Hanks balanced salt solution, almost all the infectivity and most of the radioactivity were recovered in a single band with density from 1.16 to 1.23 g/cm3 and a peak at 1.2 g/cm3. Analysis by polyacrylamide gel electrophoresis resolved the purified virus into seven polypeptides of approximate molecular weights 20,000 to 80,000, of which the two largest and the smallest proved to by glycoproteins.

Citing Articles

Respiratory syncytial virus entry and how to block it.

Battles M, McLellan J Nat Rev Microbiol. 2019; 17(4):233-245.

PMID: 30723301 PMC: 7096974. DOI: 10.1038/s41579-019-0149-x.

RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane.

Vanover D, Smith D, Blanchard E, Alonas E, Kirschman J, Lifland A Nat Commun. 2017; 8(1):667.

PMID: 28939853 PMC: 5610308. DOI: 10.1038/s41467-017-00732-z.

Identification of a Human Respiratory Syncytial Virus Cell Entry Inhibitor by Using a Novel Lentiviral Pseudotype System.

Haid S, Grethe C, Bankwitz D, Grunwald T, Pietschmann T J Virol. 2016; 90(6):3065-73.

PMID: 26719246 PMC: 4810627. DOI: 10.1128/JVI.03074-15.

Preventing Cleavage of the Respiratory Syncytial Virus Attachment Protein in Vero Cells Rescues the Infectivity of Progeny Virus for Primary Human Airway Cultures.

Corry J, Johnson S, Cornwell J, Peeples M J Virol. 2015; 90(3):1311-20.

PMID: 26581976 PMC: 4719620. DOI: 10.1128/JVI.02351-15.

Structure and function of respiratory syncytial virus surface glycoproteins.

McLellan J, Ray W, Peeples M Curr Top Microbiol Immunol. 2013; 372:83-104.

PMID: 24362685 PMC: 4211642. DOI: 10.1007/978-3-642-38919-1_4.

References
1.
Levine S, Buthala D, HAMILTON R . Late stage synchronization of respiratory syncytial virus replication. Virology. 1971; 45(2):390-400. DOI: 10.1016/0042-6822(71)90340-0. View
2.
Norrby E, Marusyk H, Orvell C . Morphogenesis of respiratory syncytial virus in a green monkey kidney cell line (Vero). J Virol. 1970; 6(2):237-42. PMC: 376113. DOI: 10.1128/JVI.6.2.237-242.1970. View
3.
Waters D, BUSSELL R . Polypeptide composition of measles and canine distemper viruses. Virology. 1973; 55(2):554-7. DOI: 10.1016/0042-6822(73)90202-x. View
4.
Berthiaume L, Joncas J, PAVILANIS V . Comparative structure, morphogenesis and biological characteristics of the respiratory syncytial (RS) virus and the pneumonia virus of mice (PVM). Arch Gesamte Virusforsch. 1974; 45(1-2):39-51. DOI: 10.1007/BF01240540. View
5.
Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View