Phenotypic Identification of Amplifications of the ADH4 and CUP1 Genes of Saccharomyces Cerevisiae

Overview

Journal Curr Genet

Specialty Genetics

Date 1993 May 1

PMID 8319294

Citations 6

Authors

M J Dorsey

P Hoeh

C E Paquin

Affiliations

Soon will be listed here.

Abstract

Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.

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