Methylation Patterns in the Human Muscle-specific Enolase Gene (ENO3)

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Jun 15

PMID 8318001

Authors

M Peshavaria

I N Day

Affiliations

Soon will be listed here.

Abstract

The methylation status in the human-muscle enolase gene (ENO3) was assayed. Previous sequence data and MspI cleavage sites indicate the presence of a 5' CpG-rich island of at least 4 kb: none of 22 characterized MspI CCGG sites is methylated in any of muscle, sperm or brain DNA. However a complex pattern of complete and partial methylation of MspI sites that is different between tissues is observed within the ENO3 gene: events at one site may be specific to muscle DNA. The absence of methylation in the promoter region of the ENO3 gene makes it unlikely that methylation plays a causal role either in transcriptional events or in the divergence of enolase-isogene regulation. The role of tissue-specific methylation events within ENO3 remains to be determined.

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