Functional Differences Between Mammalian Transcription Activation Domains at the Yeast GAL1 Promoter

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1994 Feb 1

PMID 8313909

Citations 25

Authors

M Kunzler

G H Braus

O Georgiev

K Seipel

W Schaffner

Affiliations

Soon will be listed here.

Abstract

We have fused representatives of three structurally and functionally distinct classes of mammalian transcription activation domains for RNA polymerase II to the yeast GAL4 DNA binding domain. All fusion proteins were stable when expressed in yeast and were tested for their ability to activate transcription from various positions in the yeast GAL1 promoter. Activation domains functional from remote as well as TATA-proximal positions in mammalian cells, e.g. the acidic-type domain of VP16, also stimulate transcription in yeast from various promoter positions. Proline-rich domains, as e.g. in AP-2 and CTF/NF1, with considerable promoter activity and low enhancer activity in mammalian cells stimulate transcription in yeast only from a position close to the TATA box. The glutamine-rich domains of Oct1, Oct2 and Sp1, which activate transcription in mammalian cells from close to the TATA box in response to a remote enhancer, are inactive in the yeast GAL1 promoter. This finding might reflect some basic difference between the organization of yeast and mammalian promoters.

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References

Cochran M, Weissmann C . Modular structure of the beta-globin and the TK promoters. EMBO J. 1984; 3(11):2453-9. PMC: 557711. DOI: 10.1002/j.1460-2075.1984.tb02155.x. View

Guarente L . Conservation and evolution of transcriptional mechanisms in eukaryotes. Trends Genet. 1992; 8(1):27-32. DOI: 10.1016/0168-9525(92)90021-u. View

Keegan L, Gill G, Ptashne M . Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science. 1986; 231(4739):699-704. DOI: 10.1126/science.3080805. View

Pinkham J, Olesen J, Guarente L . Sequence and nuclear localization of the Saccharomyces cerevisiae HAP2 protein, a transcriptional activator. Mol Cell Biol. 1987; 7(2):578-85. PMC: 365111. DOI: 10.1128/mcb.7.2.578-585.1987. View

Sengstag C, Hinnen A . The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual aminoacid composition. Nucleic Acids Res. 1987; 15(1):233-46. PMC: 340407. DOI: 10.1093/nar/15.1.233. View

Fink G . Pseudogenes in yeast?. Cell. 1987; 49(1):5-6. DOI: 10.1016/0092-8674(87)90746-x. View

Arndt K, Styles C, Fink G . Multiple global regulators control HIS4 transcription in yeast. Science. 1987; 237(4817):874-80. DOI: 10.1126/science.3303332. View

Gill G, Ptashne M . Mutants of GAL4 protein altered in an activation function. Cell. 1987; 51(1):121-6. DOI: 10.1016/0092-8674(87)90016-x. View

Lech K, Anderson K, Brent R . DNA-bound Fos proteins activate transcription in yeast. Cell. 1988; 52(2):179-84. DOI: 10.1016/0092-8674(88)90506-5. View

10.

Struhl K . The JUN oncoprotein, a vertebrate transcription factor, activates transcription in yeast. Nature. 1988; 332(6165):649-50. DOI: 10.1038/332649a0. View

11.

Metzger D, White J, Chambon P . The human oestrogen receptor functions in yeast. Nature. 1988; 334(6177):31-6. DOI: 10.1038/334031a0. View

12.

Schena M, Yamamoto K . Mammalian glucocorticoid receptor derivatives enhance transcription in yeast. Science. 1988; 241(4868):965-7. DOI: 10.1126/science.3043665. View

13.

Hinnebusch A . Mechanisms of gene regulation in the general control of amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Rev. 1988; 52(2):248-73. PMC: 373138. DOI: 10.1128/mr.52.2.248-273.1988. View

14.

Sadowski I, Ma J, Triezenberg S, Ptashne M . GAL4-VP16 is an unusually potent transcriptional activator. Nature. 1988; 335(6190):563-4. DOI: 10.1038/335563a0. View

15.

Muller M, Gerster T, Schaffner W . Enhancer sequences and the regulation of gene transcription. Eur J Biochem. 1988; 176(3):485-95. DOI: 10.1111/j.1432-1033.1988.tb14306.x. View

16.

Ptashne M . How eukaryotic transcriptional activators work. Nature. 1988; 335(6192):683-9. DOI: 10.1038/335683a0. View

17.

Courey A, Tjian R . Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell. 1988; 55(5):887-98. DOI: 10.1016/0092-8674(88)90144-4. View

18.

Suzuki Y, Nogi Y, Abe A, Fukasawa T . GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae. Mol Cell Biol. 1988; 8(11):4991-9. PMC: 365593. DOI: 10.1128/mcb.8.11.4991-4999.1988. View

19.

Pfeifer K, Kim K, Kogan S, Guarente L . Functional dissection and sequence of yeast HAP1 activator. Cell. 1989; 56(2):291-301. DOI: 10.1016/0092-8674(89)90903-3. View

20.

Miozzari G, Niederberger P, Hutter R . Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway. J Bacteriol. 1978; 134(1):48-59. PMC: 222216. DOI: 10.1128/jb.134.1.48-59.1978. View