Release of Bile Canalicular Membrane Antigen into Blood in Experimental Extrahepatic Cholestasis of the Rat

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 1994 Feb 1

PMID 8313808

Citations 1

Authors

Y Kobayashi

H Ohta

T Kawasaki

M Matsumoto

K Tamakoshi

K Kanai

Affiliations

Soon will be listed here.

Abstract

The level of a bile canalicular membrane antigen in serum during extrahepatic cholestasis was serially analyzed using HAM.4, a monoclonal antibody against a bile canalicular membrane glycoprotein of normal rat hepatocytes. After bile duct ligation, the level of HAM.4 antigen in serum promptly increased within 1 hr, reached a maximum at 3 hr, and declined somewhat until 48 hr, where it plateaued. Elevated levels of HAM.4 antigen in serum preceded those of well-known biliary marker enzymes activities. Immunohistochemical studies showed that the expression of HAM.4 antigen in hepatocytes and bile duct cells was not altered appreciably after bile duct ligation even when HAM.4 antigen in serum reached a maximal level. The serum and hepatic HAM.4 antigen had a molecular weight of 110 kDa. These results suggest that HAM.4 antigen may be regarded as a potential marker of the early stage of cholestasis, with release occurring before apparent histological changes.

Citing Articles

Identification of bile canalicular cell surface antigen HAM.4 as dipeptidyl peptidase IV (DPPIV) and characterization of its role in hepatic regeneration after partial hepatectomy in rats.

Tsugiki M, Kobayashi Y, Kawasaki T, Yoshimi T Dig Dis Sci. 1999; 43(12):2591-600.

PMID: 9881487 DOI: 10.1023/a:1026678622356.

References

Maurice M, Garbarz M, Feldmann G . Characterization of rat hepatocyte plasma membrane domains by monoclonal antibodies. Eur J Cell Biol. 1985; 39(1):122-9. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hubbard A, Bartles J, Braiterman L . Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies. J Cell Biol. 1985; 100(4):1115-25. PMC: 2113748. DOI: 10.1083/jcb.100.4.1115. View

Hino M, Nagatsu T, Kakumu S, Okuyama S, Yoshii Y, Nagatsu I . Glycylprolyl beta-naphthylamidase activity in human serum. Clin Chim Acta. 1975; 62(1):5-11. DOI: 10.1016/0009-8981(75)90273-9. View

Meier P . Transport polarity of hepatocytes. Semin Liver Dis. 1988; 8(4):293-307. DOI: 10.1055/s-2008-1040551. View

De Vos R, Desmet V . Morphologic changes of the junctional complex of the hepatocytes in rat liver after bile duct ligation. Br J Exp Pathol. 1978; 59(2):220-7. PMC: 2041342. View

McCaughan G, Wickson J, Creswick P, Gorrell M . Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence. Hepatology. 1990; 11(4):534-44. DOI: 10.1002/hep.1840110403. View

Ronchi G, Desmet V . Histochemical study of so-called "marker enzymes of cholestasis" during extrahepatic bile duct obstruction in the rat. Beitr Pathol. 1973; 149(3):213-6. DOI: 10.1016/s0005-8165(73)80139-8. View

Metz J, Aoki A, Merlo M, Forssmann W . Morphological alterations and functional changes of interhepatocellular junctions induced by bile duct ligation. Cell Tissue Res. 1977; 182(3):299-310. DOI: 10.1007/BF00219766. View

10.

Tamakoshi K, Fukumoto T, Kanai K, Yamashita A . A monoclonal antibody to a rat hepato-renal membrane antigen. Clin Exp Immunol. 1985; 60(2):373-80. PMC: 1577025. View

11.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

12.

Yamaguchi K, Fujikura Y, Kuniki H, Itoh K, Tamakoshi K, Fukumoto T . Immunoelectron microscopic localization of cell surface antigens on rat hepatocytes detected with monoclonal antibodies (HAM2 and HAM4). Cell Struct Funct. 1991; 16(4):303-13. DOI: 10.1247/csf.16.303. View

13.

Toda G, Kako M, Oka H, Oda T, Ikeda Y . Uneven distribution of enzymatic alterations on the liver cell surface in experimental extrahepatic cholestasis of rat. Exp Mol Pathol. 1978; 28(1):10-24. DOI: 10.1016/0014-4800(78)90059-x. View

14.

Bulle F, Mavier P, Zafrani E, Preaux A, Lescs M, Siegrist S . Mechanism of gamma-glutamyl transpeptidase release in serum during intrahepatic and extrahepatic cholestasis in the rat: a histochemical, biochemical and molecular approach. Hepatology. 1990; 11(4):545-50. DOI: 10.1002/hep.1840110404. View

15.

Berry M, Friend D . High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969; 43(3):506-20. PMC: 2107801. DOI: 10.1083/jcb.43.3.506. View

16.

Fukumoto T, Tamakoshi K, Ohta H, Yamashita A, Maeda K . Purification and characterisation of MHC class I antigen from rat liver with monoclonal antibody. Int J Biochem. 1986; 18(11):971-7. DOI: 10.1016/0020-711x(86)90241-7. View

17.

Maurice M, Dumont M, Feldmann G . Effect of colchicine and phalloidin on the distribution of three plasma membrane antigens in rat hepatocytes: comparison with bile duct ligation. Hepatology. 1987; 7(6):1239-48. DOI: 10.1002/hep.1840070611. View

18.

Felker T, Hamilton R, HAVEL R . Secretion of lipoprotein-X by perfused livers of rats with cholestasis. Proc Natl Acad Sci U S A. 1978; 75(7):3459-63. PMC: 392797. DOI: 10.1073/pnas.75.7.3459. View

19.

Moreau A, Maurice M, Feldmann G . Analysis of hepatocyte plasma membrane domains during rat development using monoclonal antibodies. J Histochem Cytochem. 1988; 36(1):87-94. DOI: 10.1177/36.1.3275714. View

20.

Easter D, Wade J, Boyer J . Structural integrity of hepatocyte tight junctions. J Cell Biol. 1983; 96(3):745-9. PMC: 2112395. DOI: 10.1083/jcb.96.3.745. View