Establishment of a Normal Range of Morphometric Values for Peroxisomes in Paediatric Liver

Overview

Journal Virchows Arch A Pathol Anat Histopathol

Specialty Anatomy & Histology

Date 1993 Jan 1

PMID 8291218

Citations 3

Authors

J L Hughes

A J Bourne

A Poulos

Affiliations

Soon will be listed here.

Abstract

The size and number of hepatic peroxisomes was investigated in 16 control paediatric liver biopsies from patients ranging in age from 3 months to 18 years one fetal liver specimen and one paediatric autopsy liver. The area, diameter, volume density (Vv), numerical density (Nv) and surface density (Sv) of the peroxisomes was recorded using randomly selected electron micrographs. The mean diameter of peroxisomes in control paediatric liver was 0.56 microns, the mean Vv was 1.67%, the mean Nv was 0.125 per micron+3 and the mean Sv was 0.161 per micron. No correlation was found between the size and number of hepatic peroxisomes and the age or sex of the patient. Peroxisomes in the fetal liver were smaller than those in biopsy tissue and had a mean diameter of 0.42 micron. Peroxisomes were identified in autopsy tissue and were enlarged with a mean diameter of 0.75 micron, most probably due to post-mortem swelling. A range of morphometric values in paediatric liver has now been established.

Citing Articles

Secondary alterations of human hepatocellular peroxisomes.

De Craemer D J Inherit Metab Dis. 1995; 18 Suppl 1:181-213.

PMID: 9053551 DOI: 10.1007/BF00711439.

Practical guide for morphometry of human peroxisomes on electron micrographs.

Kerckaert I, De Craemer D, Van Limbergen G J Inherit Metab Dis. 1995; 18 Suppl 1:172-80.

PMID: 9053550 DOI: 10.1007/BF00711438.

Morphometry of peroxisomes and immunolocalization of peroxisomal proteins in the liver of patients with generalised peroxisomal disorders.

Hughes J, Crane D, Robertson E, Poulos A Virchows Arch A Pathol Anat Histopathol. 1993; 423(6):459-68.

PMID: 7507276 DOI: 10.1007/BF01606536.

References

Hughes J, Poulos A, Robertson E, Chow C, Sheffield L, Christodoulou J . Pathology of hepatic peroxisomes and mitochondria in patients with peroxisomal disorders. Virchows Arch A Pathol Anat Histopathol. 1990; 416(3):255-64. DOI: 10.1007/BF01678985. View

Roels F, Cornelis A, Poll-The B, Aubourg P, Ogier H, Scotto J . Hepatic peroxisomes are deficient in infantile refsum disease: a cytochemical study of 4 cases. Am J Med Genet. 1986; 25(2):257-71. DOI: 10.1002/ajmg.1320250210. View

KANAMURA S, Kanai K, Watanabe J . Fine structure and function of hepatocytes during development. J Electron Microsc Tech. 1990; 14(2):92-105. DOI: 10.1002/jemt.1060140204. View

Moser H, Bergin A, Cornblath D . Peroxisomal disorders. Biochem Cell Biol. 1991; 69(7):463-74. DOI: 10.1139/o91-070. View

ROHR H, Luthy J, Gudat F, Oberholzer M, Gysin C, Bianchi L . Stereology of liver biopsies from healthy volunteers. Virchows Arch A Pathol Anat Histol. 1976; 371(3):251-63. DOI: 10.1007/BF00433072. View

NOVIKOFF A, Goldfischer S . Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J Histochem Cytochem. 1969; 17(10):675-80. DOI: 10.1177/17.10.675. View

Roels F, Pauwels M, Poll-The B, Scotto J, Ogier H, Aubourg P . Hepatic peroxisomes in adrenoleukodystrophy and related syndromes: cytochemical and morphometric data. Virchows Arch A Pathol Anat Histopathol. 1988; 413(4):275-85. DOI: 10.1007/BF00783019. View

De Craemer D, Zweens M, Lyonnet S, Wanders R, Poll-The B, Schutgens R . Very large peroxisomes in distinct peroxisomal disorders (rhizomelic chondrodysplasia punctata and acyl-CoA oxidase deficiency): novel data. Virchows Arch A Pathol Anat Histopathol. 1991; 419(6):523-5. DOI: 10.1007/BF01650683. View

GIESECKE D, Pospischil A, Laging C, Heiss G . Postnatal development of hepatic uricase and peroxisomes in baby pigs. Comp Biochem Physiol B. 1987; 88(3):999-1003. DOI: 10.1016/0305-0491(87)90276-8. View

10.

Poll-The B, Roels F, Ogier H, Scotto J, Vamecq J, Schutgens R . A new peroxisomal disorder with enlarged peroxisomes and a specific deficiency of acyl-CoA oxidase (pseudo-neonatal adrenoleukodystrophy). Am J Hum Genet. 1988; 42(3):422-34. PMC: 1715143. View

11.

Hughes J, Poulos A, Crane D, Chow C, Sheffield L, Sillence D . Ultrastructure and immunocytochemistry of hepatic peroxisomes in rhizomelic chondrodysplasia punctata. Eur J Pediatr. 1992; 151(11):829-36. DOI: 10.1007/BF01957935. View

12.

Briere N . Peroxisomes in human foetal kidney: variations in size and number during development. Anat Embryol (Berl). 1986; 174(2):235-42. DOI: 10.1007/BF00824339. View

13.

Espeel M, Jauniaux E, Hashimoto T, Roels F . Immunocytochemical localization of peroxisomal beta-oxidation enzymes in human fetal liver. Prenat Diagn. 1990; 10(6):349-57. DOI: 10.1002/pd.1970100602. View

14.

JEZEQUEL A, Librari M, Mosca P, Novelli G . The human liver in extrahepatic cholestasis: ultrastructural morphometric data. Liver. 1983; 3(5):303-14. DOI: 10.1111/j.1600-0676.1983.tb00882.x. View

15.

Schmucker D . Hepatocyte fine structure during maturation and senescence. J Electron Microsc Tech. 1990; 14(2):106-25. DOI: 10.1002/jemt.1060140205. View

16.

Roessner A, Kolde G, Stahl K, Blanke G, van Husen N, Themann H . Ultrastructural morphometric investigations on normal human liver biopsies. Acta Hepatogastroenterol (Stuttg). 1978; 25(2):119-23. View

17.

Stefanini S, Farrace M, ARGENTO M . Differentiation of liver peroxisomes in the foetal and newborn rat. Cytochemistry of catalase and D-aminoacid oxidase. J Embryol Exp Morphol. 1985; 88:151-63. View

18.

Roels F, Wisse E, De Prest B, van der Meulen J . Cytochemical discrimination between catalases and peroxidases using diaminobenzidine. Histochemistry. 1975; 41(4):281-312. DOI: 10.1007/BF00490073. View

19.

Sternlieb I, Quintana N . The peroxisomes of human hepatocytes. Lab Invest. 1977; 36(2):140-9. View

20.

Beier K, Fahimi H . Application of automatic image analysis for quantitative morphological studies of peroxisomes in rat liver in conjunction with cytochemical staining with 3-3'-diaminobenzidine and immunocytochemistry. Microsc Res Tech. 1992; 21(4):271-82. DOI: 10.1002/jemt.1070210404. View