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Detection of Cryptosporidium Parvum Using a Specific Polymerase Chain Reaction

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Journal Vet Parasitol
Publisher Elsevier
Date 1993 Oct 1
PMID 8291195
Citations 18
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Abstract

The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.

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