» Articles » PMID: 8288911

Epidermis As a Secretory Tissue: an in Vitro Tissue Model to Study Keratinocyte Secretion

Overview
Publisher Elsevier
Specialty Dermatology
Date 1994 Jan 1
PMID 8288911
Citations 10
Authors
Affiliations
Soon will be listed here.
Abstract

In addition to protective functions, keratinocytes secrete a variety of effector molecules that may have local or distant effects. To explore the secretory activity of keratinocytes we have developed a two-chamber culture model in which a fully differentiated stratified epithelium is present in the upper chamber and secreted protein is collected in a lower chamber. A collagen matrix is not used and during collection of secreted protein, 3T3 feeder cells are absent. Keratinocytes secrete protein at the rate of 0.67 microgram/h/10(6) cells, encompassing a molecular weight range from several kD to over 180 kD. Secretion is an unexplored area of keratinocyte biology and this model will allow investigation of this activity.

Citing Articles

Biogenesis of specialized lysosomes in differentiated keratinocytes relies on close apposition with the Golgi apparatus.

Mahanty S, Bergam P, Belapurkar V, Eluvathingal L, Gupta N, Goud B Cell Death Dis. 2024; 15(7):496.

PMID: 38992005 PMC: 11239851. DOI: 10.1038/s41419-024-06710-w.


Current and Emerging Topical Scar Mitigation Therapies for Craniofacial Burn Wound Healing.

Kwon S, Barrera J, Noishiki C, Chen K, Henn D, Sheckter C Front Physiol. 2020; 11:916.

PMID: 32848859 PMC: 7403506. DOI: 10.3389/fphys.2020.00916.


Cultured Human Epidermis Combined With Meshed Skin Autografts Accelerates Epithelialization and Granulation Tissue Formation in a Rat Model.

Sakamoto M, Morimoto N, Inoie M, Takahagi M, Ogino S, Jinno C Ann Plast Surg. 2017; 78(6):651-658.

PMID: 28230648 PMC: 5434968. DOI: 10.1097/SAP.0000000000001058.


Epidermal micrografts produced via an automated and minimally invasive tool form at the dermal/epidermal junction and contain proliferative cells that secrete wound healing growth factors.

Osborne S, Schmidt M, Derrick K, Harper J Adv Skin Wound Care. 2015; 28(9):397-405.

PMID: 26258460 PMC: 4892759. DOI: 10.1097/01.ASW.0000470024.81711.b8.


What you always needed to know about electroporation based DNA vaccines.

Gothelf A, Gehl J Hum Vaccin Immunother. 2012; 8(11):1694-702.

PMID: 23111168 PMC: 3601144. DOI: 10.4161/hv.22062.