Epidermis As a Secretory Tissue: an in Vitro Tissue Model to Study Keratinocyte Secretion
Overview
Authors
Affiliations
In addition to protective functions, keratinocytes secrete a variety of effector molecules that may have local or distant effects. To explore the secretory activity of keratinocytes we have developed a two-chamber culture model in which a fully differentiated stratified epithelium is present in the upper chamber and secreted protein is collected in a lower chamber. A collagen matrix is not used and during collection of secreted protein, 3T3 feeder cells are absent. Keratinocytes secrete protein at the rate of 0.67 microgram/h/10(6) cells, encompassing a molecular weight range from several kD to over 180 kD. Secretion is an unexplored area of keratinocyte biology and this model will allow investigation of this activity.
Mahanty S, Bergam P, Belapurkar V, Eluvathingal L, Gupta N, Goud B Cell Death Dis. 2024; 15(7):496.
PMID: 38992005 PMC: 11239851. DOI: 10.1038/s41419-024-06710-w.
Current and Emerging Topical Scar Mitigation Therapies for Craniofacial Burn Wound Healing.
Kwon S, Barrera J, Noishiki C, Chen K, Henn D, Sheckter C Front Physiol. 2020; 11:916.
PMID: 32848859 PMC: 7403506. DOI: 10.3389/fphys.2020.00916.
Sakamoto M, Morimoto N, Inoie M, Takahagi M, Ogino S, Jinno C Ann Plast Surg. 2017; 78(6):651-658.
PMID: 28230648 PMC: 5434968. DOI: 10.1097/SAP.0000000000001058.
Osborne S, Schmidt M, Derrick K, Harper J Adv Skin Wound Care. 2015; 28(9):397-405.
PMID: 26258460 PMC: 4892759. DOI: 10.1097/01.ASW.0000470024.81711.b8.
What you always needed to know about electroporation based DNA vaccines.
Gothelf A, Gehl J Hum Vaccin Immunother. 2012; 8(11):1694-702.
PMID: 23111168 PMC: 3601144. DOI: 10.4161/hv.22062.