Coexpression of Eukaryotic TRNASer and Yeast Seryl-tRNA Synthetase Leads to Functional Amber Suppression in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 Jan 1

PMID 8282701

Citations 4

Authors

M Nalaskowska

D Soll

Affiliations

Soon will be listed here.

Abstract

In order to gain insight into the conservation of determinants for tRNA identity between organisms, Schizosaccharomyces pombe and human amber suppressor serine tRNA genes have been examined for functional expression in Escherichia coli. The primary transcripts, which originated from E. coli plasmid promoters, were processed into mature tRNAs, but they were poorly aminoacylated in E. coli and thus were nonfunctional as suppressors in vivo. However, coexpression of cloned Saccharomyces cerevisiae seryl-tRNA synthetase led to efficient suppression in E. coli. This shows that some, but not all, determinants specifying the tRNASer identity are conserved in evolution.

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References

Sampson J, DiRenzo A, Behlen L, Uhlenbeck O . Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase. Science. 1989; 243(4896):1363-6. DOI: 10.1126/science.2646717. View

Racher K, Kalmar G, Borgford T . Expression and characterization of a recombinant yeast isoleucyl-tRNA synthetase. J Biol Chem. 1991; 266(26):17158-64. View

Dock-Bregeon A, Westhof E, Giege R, Moras D . Solution structure of a tRNA with a large variable region: yeast tRNASer. J Mol Biol. 1989; 206(4):707-22. DOI: 10.1016/0022-2836(89)90578-0. View

Normanly J, Abelson J . tRNA identity. Annu Rev Biochem. 1989; 58:1029-49. DOI: 10.1146/annurev.bi.58.070189.005121. View

Hou Y, Schimmel P . Evidence that a major determinant for the identity of a transfer RNA is conserved in evolution. Biochemistry. 1989; 28(17):6800-4. DOI: 10.1021/bi00443a003. View

Dock-Bregeon A, Garcia A, Giege R, Moras D . The contacts of yeast tRNA(Ser) with seryl-tRNA synthetase studied by footprinting experiments. Eur J Biochem. 1990; 188(2):283-90. DOI: 10.1111/j.1432-1033.1990.tb15401.x. View

Edwards H, Schimmel P . A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase. Mol Cell Biol. 1990; 10(4):1633-41. PMC: 362268. DOI: 10.1128/mcb.10.4.1633-1641.1990. View

Himeno H, Hasegawa T, Ueda T, Watanabe K, Shimizu M . Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro. Nucleic Acids Res. 1990; 18(23):6815-9. PMC: 332736. DOI: 10.1093/nar/18.23.6815. View

Edwards H, Trezeguet V, Schimmel P . An Escherichia coli tyrosine transfer RNA is a leucine-specific transfer RNA in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991; 88(4):1153-6. PMC: 50975. DOI: 10.1073/pnas.88.4.1153. View

10.

Schatz D, Leberman R, Eckstein F . Interaction of Escherichia coli tRNA(Ser) with its cognate aminoacyl-tRNA synthetase as determined by footprinting with phosphorothioate-containing tRNA transcripts. Proc Natl Acad Sci U S A. 1991; 88(14):6132-6. PMC: 52036. DOI: 10.1073/pnas.88.14.6132. View

11.

Sundharadas G, Katze J, Soll D, Konigsberg W, Lengyel P . On the recognition of serine transfer RNA's specific for unrelated codons by the same seryl-transfer RNA synthetase. Proc Natl Acad Sci U S A. 1968; 61(2):693-700. PMC: 225215. DOI: 10.1073/pnas.61.2.693. View

12.

Jahn M, Rogers M, Soll D . Anticodon and acceptor stem nucleotides in tRNA(Gln) are major recognition elements for E. coli glutaminyl-tRNA synthetase. Nature. 1991; 352(6332):258-60. DOI: 10.1038/352258a0. View

13.

Varshney U, Lee C, RajBhandary U . Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase. J Biol Chem. 1991; 266(36):24712-8. View

14.

Lee C, RajBhandary U . Mutants of Escherichia coli initiator tRNA that suppress amber codons in Saccharomyces cerevisiae and are aminoacylated with tyrosine by yeast extracts. Proc Natl Acad Sci U S A. 1991; 88(24):11378-82. PMC: 53138. DOI: 10.1073/pnas.88.24.11378. View

15.

Rogers M, Adachi T, Inokuchi H, Soll D . Switching tRNA(Gln) identity from glutamine to tryptophan. Proc Natl Acad Sci U S A. 1992; 89(8):3463-7. PMC: 48888. DOI: 10.1073/pnas.89.8.3463. View

16.

Normanly J, Ollick T, Abelson J . Eight base changes are sufficient to convert a leucine-inserting tRNA into a serine-inserting tRNA. Proc Natl Acad Sci U S A. 1992; 89(12):5680-4. PMC: 49356. DOI: 10.1073/pnas.89.12.5680. View

17.

Sherman J, Rogers M, Soll D . Competition of aminoacyl-tRNA synthetases for tRNA ensures the accuracy of aminoacylation. Nucleic Acids Res. 1992; 20(11):2847-52. PMC: 336931. DOI: 10.1093/nar/20.11.2847. View

18.

Hayase Y, Jahn M, Rogers M, Sylvers L, Koizumi M, Inoue H . Recognition of bases in Escherichia coli tRNA(Gln) by glutaminyl-tRNA synthetase: a complete identity set. EMBO J. 1992; 11(11):4159-65. PMC: 556926. DOI: 10.1002/j.1460-2075.1992.tb05509.x. View

19.

Sylvers L, Rogers K, Shimizu M, Ohtsuka E, Soll D . A 2-thiouridine derivative in tRNAGlu is a positive determinant for aminoacylation by Escherichia coli glutamyl-tRNA synthetase. Biochemistry. 1993; 32(15):3836-41. DOI: 10.1021/bi00066a002. View

20.

Ban N, Jahn D, Soll D . Yeast seryl-tRNA synthetase expressed in Escherichia coli recognizes bacterial serine-specific tRNAs in vivo. Eur J Biochem. 1993; 214(3):869-77. DOI: 10.1111/j.1432-1033.1993.tb17990.x. View