» Articles » PMID: 8282102

Analysis of Filamin and Alpha-actinin Binding to Actin by the Stopped Flow Method

Overview
Journal FEBS Lett
Specialty Biochemistry
Date 1993 Dec 28
PMID 8282102
Citations 24
Authors
Affiliations
Soon will be listed here.
Abstract

We ascertained by the stopped flow method the overall association rate constant, k+1, of filamin and alpha-actinin to fluorescently labelled filamentous actin of approximately 1.3 x 10(6) M-1.s-1 and approximately 1.0 x 10(6) M-1.s-1 as well as the overall dissociation rate constant, k-1, of approximately 0.6 s-1 and approximately 0.4 s-1, respectively. The overall equilibrium constant, K, for filamin and alpha-actinin to actin deduced from the relation K = k+1/k-1 agree well with published data.

Citing Articles

From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.

Katsuta H, Sokabe M, Hirata H Front Cell Dev Biol. 2024; 12:1444827.

PMID: 39193363 PMC: 11347286. DOI: 10.3389/fcell.2024.1444827.


α-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytes.

Tseng C, Zheng R, Lin T, Chou C, Shih Y, Liang S Life Sci Alliance. 2022; 5(11).

PMID: 36096674 PMC: 9468603. DOI: 10.26508/lsa.202201557.


Protein friction and filament bending facilitate contraction of disordered actomyosin networks.

Tam A, Mogilner A, Oelz D Biophys J. 2021; 120(18):4029-4040.

PMID: 34390686 PMC: 8510976. DOI: 10.1016/j.bpj.2021.08.012.


Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.

Anderson S, Garamella J, Adalbert S, McGorty R, Robertson-Anderson R Soft Matter. 2021; 17(16):4375-4385.

PMID: 33908593 PMC: 8189643. DOI: 10.1039/d1sm00093d.


Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.

Chaubet L, Chaudhary A, Heris H, Ehrlicher A, Hendricks A Mol Biol Cell. 2020; 31(16):1744-1752.

PMID: 32579489 PMC: 7521843. DOI: 10.1091/mbc.E19-09-0504.