Temperature-sensitive Steps in the Transport of Semliki Forest Virus Envelope Proteins in Mosquito C6/36 Cells

Overview

Journal Arch Virol

Specialty Microbiology

Date 1994 Jan 1

PMID 8279948

Authors

C Vallan

C G Scharer

H KOBLET

Affiliations

Soon will be listed here.

Abstract

We have analysed the temperature dependence of the transport of Semliki Forest virus (SFV) envelope proteins in mosquito cells, the natural host cells of alphaviruses. These cells are cultivated at a lower temperature (28 degrees C) and have a different lipid composition as compared to mammalian cells. When the incubation temperature was reduced at early times after infection, the onset of virus shedding was delayed and the maximal titers decreased correspondingly to the temperature. No virus was shed at 12 degrees C. No evidence was observed for a block of virus release due to a shift of the sites of virus maturation. When the incubation temperature was reduced at later times after infection a critical temperature of 12 degrees C was again observed. At this temperature no transport of viral proteins took place, p62 remained uncleaved, the glycan processing of E1 did not occur and the envelope proteins accumulated in a pre-Golgi compartment. We suggest a mathematical formula which allows the extrapolation of transport data to the temperature at which intracellular protein transport becomes blocked.

References

Kuismanen E, Saraste J . Low temperature-induced transport blocks as tools to manipulate membrane traffic. Methods Cell Biol. 1989; 32:257-74. DOI: 10.1016/s0091-679x(08)61174-7. View

Rotundo R, Fambrough D . Secretion of acetylcholinesterase: relation to acetylcholine receptor metabolism. Cell. 1980; 22(2 Pt 2):595-602. DOI: 10.1016/0092-8674(80)90369-4. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Braakman I, Wagner K, Helenius A . Folding of influenza hemagglutinin in the endoplasmic reticulum. J Cell Biol. 1991; 114(3):401-11. PMC: 2289100. DOI: 10.1083/jcb.114.3.401. View

Chamberlain J . Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979; 98(1):132-5. DOI: 10.1016/0003-2697(79)90716-4. View

Naim H, KOBLET H . The cleavage of p62, the precursor of E2 and E3, is an early and continuous event in Semliki Forest virus-infected Aedes albopictus cells. Arch Virol. 1990; 110(3-4):221-37. DOI: 10.1007/BF01311290. View

Zhao H, Garoff H . Role of cell surface spikes in alphavirus budding. J Virol. 1992; 66(12):7089-95. PMC: 240383. DOI: 10.1128/JVI.66.12.7089-7095.1992. View

Bordier C . Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem. 1981; 256(4):1604-7. View

Russell D, Dalrymple J, Johnston R . Sindbis virus mutations which coordinately affect glycoprotein processing, penetration, and virulence in mice. J Virol. 1989; 63(4):1619-29. PMC: 248406. DOI: 10.1128/JVI.63.4.1619-1629.1989. View

10.

Saraste J, Palade G, Farquhar M . Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells. Proc Natl Acad Sci U S A. 1986; 83(17):6425-9. PMC: 386516. DOI: 10.1073/pnas.83.17.6425. View

11.

Igarashi A . Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol. 1978; 40(3):531-44. DOI: 10.1099/0022-1317-40-3-531. View

12.

Raghow R, GRACE T, Filshie B, Bartley W, Dalgarno L . Ross River virus replication in cultured mosquito and mammalian cells: virus growth and correlated ultrastructural changes. J Gen Virol. 1973; 21:109-22. DOI: 10.1099/0022-1317-21-1-109. View

13.

Saraste J, Kuismanen E . Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface. Cell. 1984; 38(2):535-49. DOI: 10.1016/0092-8674(84)90508-7. View

14.

Liljestrom P, Garoff H . Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor. J Virol. 1991; 65(1):147-54. PMC: 240499. DOI: 10.1128/JVI.65.1.147-154.1991. View

15.

Presley J, Polo J, Johnston R, Brown D . Proteolytic processing of the Sindbis virus membrane protein precursor PE2 is nonessential for growth in vertebrate cells but is required for efficient growth in invertebrate cells. J Virol. 1991; 65(4):1905-9. PMC: 240008. DOI: 10.1128/JVI.65.4.1905-1909.1991. View

16.

Ceriotti A, Colman A . Trimer formation determines the rate of influenza virus haemagglutinin transport in the early stages of secretion in Xenopus oocytes. J Cell Biol. 1990; 111(2):409-20. PMC: 2116180. DOI: 10.1083/jcb.111.2.409. View

17.

Naim H, KOBLET H . Asparagine-linked oligosaccharides of Semliki Forest virus grown in mosquito cells. Arch Virol. 1992; 122(1-2):45-60. DOI: 10.1007/BF01321117. View

18.

van t Hof W, van Meer G . Generation of lipid polarity in intestinal epithelial (Caco-2) cells: sphingolipid synthesis in the Golgi complex and sorting before vesicular traffic to the plasma membrane. J Cell Biol. 1990; 111(3):977-86. PMC: 2116282. DOI: 10.1083/jcb.111.3.977. View

19.

KORNFELD R, Kornfeld S . Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985; 54:631-64. DOI: 10.1146/annurev.bi.54.070185.003215. View

20.

Gliedman J, Smith J, Brown D . Morphogenesis of Sindbis virus in cultured Aedes albopictus cells. J Virol. 1975; 16(4):913-26. PMC: 354753. DOI: 10.1128/JVI.16.4.913-926.1975. View