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Formation of Dry-heat Resistant Bacillus Subtilis Var. Niger Spores As Influenced by the Composition of the Sporulation Medium

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Publisher Springer
Specialty Microbiology
Date 1976 Jan 1
PMID 827974
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Abstract

Bacillus subtilis var. niger spores were produced on 20 different media. The spore yield from each medium and the dry-heat resistance at 160 C of the different spore populations were determined. The yield varied with a factor of 10(6) and the variation in D 160-value was about 10-fold (less than 20 S-190 S). A "synthetic" medium producing a high yield of spores with high dry-heat resistance was formulated. The concentrations of glucose, sucrose and calcium were found to be critical.

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