Cloning and Characterisation of the Cytochrome C Gene of Aspergillus Nidulans

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1994 Jan 1

PMID 8277943

Citations 3

Authors

D C Raitt

R E Bradshaw

T M Pillar

Affiliations

Soon will be listed here.

Abstract

The cytochrome c gene (cycA) of the filamentous fungus Aspergillus nidulans has been isolated and sequenced. The gene is present in a single copy per haploid genome and encodes a polypeptide of 112 amino acid residues. The nucleotide sequence of the A. nidulans cycA gene shows 87% identity to the DNA sequence of the Neurospora crassa cytochrome c gene, and approximately 72% identity to the sequence of the Saccharomyces cerevisiae iso-1-cytochrome c gene (CYC1). The S. cerevisiae CYC1 gene was used as a heterologous probe to isolate the homologous gene in A. nidulans. The A. nidulans cytochrome c sequence contains two small introns. One of these is highly conserved in terms of position, but the other has not been reported in any of the cytochrome c genes so far sequenced. Expression of the cycA gene is not affected by glucose repression, but has been shown to be induced approximately tenfold in the presence of oxygen and three- to fourfold under heat-shock conditions.

Citing Articles

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Genome-wide analysis of differentially expressed genes from Penicillium chrysogenum grown with a repressing or a non-repressing carbon source.

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Regulation of freA, acoA, lysF, and cycA expression by iron availability in Aspergillus nidulans.

Oberegger H, Schoeser M, Zadra I, Schrettl M, Parson W, Haas H Appl Environ Microbiol. 2002; 68(11):5769-72.

PMID: 12406779 PMC: 129941. DOI: 10.1128/AEM.68.11.5769-5772.2002.

References

Zitomer R, Sellers J, McCarter D, Hastings G, Wick P, Lowry C . Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene. Mol Cell Biol. 1987; 7(6):2212-20. PMC: 365345. DOI: 10.1128/mcb.7.6.2212-2220.1987. View

Pontecorvo G, Roper J, HEMMONS L, MacDonald K, BUFTON A . The genetics of Aspergillus nidulans. Adv Genet. 1953; 5:141-238. DOI: 10.1016/s0065-2660(08)60408-3. View

Pillar T, Bradshaw R . Heat shock and stationary phase induce transcription of the Saccharomyces cerevisiae iso-2 cytochrome c gene. Curr Genet. 1991; 20(3):185-8. DOI: 10.1007/BF00326230. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Pfeifer K, Prezant T, Guarente L . Yeast HAP1 activator binds to two upstream activation sites of different sequence. Cell. 1987; 49(1):19-27. DOI: 10.1016/0092-8674(87)90751-3. View

MARGOLIASH E, SCHEJTER A . Cytochrome c. Adv Protein Chem. 1966; 21:113-286. DOI: 10.1016/s0065-3233(08)60128-x. View

Lindquist S, Craig E . The heat-shock proteins. Annu Rev Genet. 1988; 22:631-77. DOI: 10.1146/annurev.ge.22.120188.003215. View

Verdiere J, Creusot F, Guarente L, SLONIMSKI P . The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c. Curr Genet. 1986; 10(5):339-42. DOI: 10.1007/BF00418404. View

Kelly J, Drysdale M, Sealy-Lewis H, JONES I, Lockington R . Alcohol dehydrogenase III in Aspergillus nidulans is anaerobically induced and post-transcriptionally regulated. Mol Gen Genet. 1990; 222(2-3):323-8. DOI: 10.1007/BF00633836. View

10.

Mattoon J, Caravajal E, GUTHRIE D . Effects of hap mutations on heme and cytochrome formation in yeast. Curr Genet. 1990; 17(2):179-83. DOI: 10.1007/BF00312865. View

11.

Waldron C, Roberts C . Cytoplasmic inheritance of a cold-sensitive mutant in Aspergillus nidulans. J Gen Microbiol. 1973; 78(2):379-81. DOI: 10.1099/00221287-78-2-379. View

12.

Kemmerer E, Lei M, Wu R . Structure and molecular evolutionary analysis of a plant cytochrome c gene: surprising implications for Arabidopsis thaliana. J Mol Evol. 1991; 32(3):227-37. DOI: 10.1007/BF02342745. View

13.

Fiechter A, FUHRMANN G, Kappeli O . Regulation of glucose metabolism in growing yeast cells. Adv Microb Physiol. 1981; 22:123-83. DOI: 10.1016/s0065-2911(08)60327-6. View

14.

Forsburg S, Guarente L . Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site. Mol Cell Biol. 1988; 8(2):647-54. PMC: 363190. DOI: 10.1128/mcb.8.2.647-654.1988. View

15.

Courgeon A, Rollet E, Becker J, Maisonhaute C . Hydrogen peroxide (H2O2) induces actin and some heat-shock proteins in Drosophila cells. Eur J Biochem. 1988; 171(1-2):163-70. DOI: 10.1111/j.1432-1033.1988.tb13772.x. View

16.

Korb H, Neupert W . Biogenesis of cytochrome c in Neurospora crassa. Synthesis of apocytochrome c, transfer to mitochondria and conversion to Holocytochrome c. Eur J Biochem. 1978; 91(2):609-20. DOI: 10.1111/j.1432-1033.1978.tb12714.x. View

17.

Sherman F, Stewart J . Genetics and biosynthesis of cytochrome c. Annu Rev Genet. 1971; 5:257-96. DOI: 10.1146/annurev.ge.05.120171.001353. View

18.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

19.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

20.

Nye S, Scarpulla R . Mitochondrial targeting of yeast apoiso-1-cytochrome c is mediated through functionally independent structural domains. Mol Cell Biol. 1990; 10(11):5763-71. PMC: 361352. DOI: 10.1128/mcb.10.11.5763-5771.1990. View