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Elemental Analysis of Renal Slices by Proton-induced X-ray Emission

Overview
Journal Environ Health Perspect
Date 1993 Sep 1
PMID 8275986
Citations 8
Authors
T Lowe
Q Chen
Q Fernando
R Keith
A J Gandolfi
Affiliations
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Abstract

We optimized proton-induced X-ray emission (PIXE) for tissue analysis in a toxicity-disposition study. We used cultured rabbit renal slices as the biological system to demonstrate the use of PIXE analysis. The renal slices were exposed to HgCl2, CdCl2, K2Cr2O7, or NaAsO2 alone or in a mixture. The PIXE analysis provides information on concentrations of elements above atomic number 11, and it is the only analytical technique that can determine 20-30 elements nondestructively in a single, small sample (approximately 5 mg) with detection limits of 1-5 ppm (dry weight). The renal slices are thin targets that yield X-ray emission spectra with low backgrounds and high elemental sensitivities. The nondestructive nature of PIXE and the ability to simultaneously measure uptake of multiple metals and endogenous elements are unique to this methodology.

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References
1.
LeFurgey A, Ingram P . Calcium measurements with electron probe X-ray and electron energy loss analysis. Environ Health Perspect. 1990; 84:57-73. PMC: 1567655. DOI: 10.1289/ehp.908457. View
2.
Maenhaut W . Multielement analysis of biological materials by particle-induced X-ray emission (PIXE). Scanning Microsc. 1990; 4(1):43-59; discussion 59-62. View
3.
Walter R, Willis R, Gutknecht W, Joyce J . Analysis of biological, clinical, and environmental samples using proton-induced X-ray emission. Anal Chem. 1974; 46(7):843-55. DOI: 10.1021/ac60343a038. View
4.
Phelps J, Gandolfi A, Brendel K, Dorr R . Cisplatin nephrotoxicity: in vitro studies with precision-cut rabbit renal cortical slices. Toxicol Appl Pharmacol. 1987; 90(3):501-12. DOI: 10.1016/0041-008x(87)90142-6. View
5.
Pinheiro T, Duflou H, Maenhaut W . Applicability of microwave acid digestion to sample preparation of biological materials for analysis by particle-induced X-ray emission (PIXE). Biol Trace Elem Res. 1990; 26-27:589-97. DOI: 10.1007/BF02992715. View