Evaluation of the Genotoxic, Cytotoxic, and Antitumor Properties of Commiphora Molmol Using Normal and Ehrlich Ascites Carcinoma Cell-bearing Swiss Albino Mice

Overview

Journal Cancer Chemother Pharmacol

Specialty Oncology

Date 1993 Jan 1

PMID 8261571

Citations 7

Authors

S Qureshi

M M Al-Harbi

M M Ahmed

M Raza

A B Giangreco

A H Shah

Affiliations

Soon will be listed here.

Abstract

The genotoxic, cytotoxic and antitumor properties of Commiphora molmol (oleo gum resin) were studied in normal and Ehrlich ascites carcinoma cell-bearing mice. In normal mice, the genotoxic and cytotoxic activity was evaluated on the bases of the frequency of micronuclei and the ratio of polychromatic to normochromatic cells in bone marrow, which was substantiated by the biochemical changes in hepatic cells. The antitumor activity of C. molmol was evaluated from the total count and viability of Ehrlich ascites carcinoma cells and their nucleic acid, protein, malondialdehyde, and elemental concentrations in addition to observations on survival and the trend of changes in body weight. The tumors at the site of injection were evaluated for histopathological changes. Treatment with C. molmol (125-500 mg/kg) showed no clastogenicity but was found to be highly cytotoxic in normal mice. The results obtained in the Ehrlich ascites carcinoma cell-bearing mice revealed the cytotoxic and antitumor activity of C. molmol which was found to be equivalent to those of the standard cytotoxic drug cyclophosphamide. On the basis of the nonmutagenic, antioxidative, and cytotoxic potential of C. molmol as observed in the present study, its use in cancer therapy seems to be appropriate and further investigations are suggested.

Citing Articles

Efficacy of myrrh extract against Eimeria labbeana-like experimental infection in Columba livia domestica: in vivo study.

Albasyouni S, Alharbi A, Al-Shaebi E, Al Quraishy S, Abdel-Gaber R BMC Vet Res. 2024; 20(1):586.

PMID: 39736719 PMC: 11684283. DOI: 10.1186/s12917-024-04450-9.

Evaluation of anthelminthic and antiprotozoal activity of myrrh () methanolic extract.

Albasyouni S, Al-Quraishy S, Al-Hoshani N, Al-Otaibi T, Al-Shaebi E, Abdel-Gaber R Helminthologia. 2024; 61(1):1-10.

PMID: 38659472 PMC: 11038267. DOI: 10.2478/helm-2024-0008.

Efficacy of myrrh mouthwash on early wound healing after tooth extraction: A randomized controlled trial.

Eid R Saudi Dent J. 2021; 33(1):44-54.

PMID: 33473242 PMC: 7801243. DOI: 10.1016/j.sdentj.2019.11.011.

attenuates oxidative and inflammatory processes in acetic acid-induced ulcerative colitis.

Fatani A, Alrojayee F, Parmar M, Abuohashish H, Ahmed M, Al-Rejaie S Exp Ther Med. 2016; 12(2):730-738.

PMID: 27446268 PMC: 4950638. DOI: 10.3892/etm.2016.3398.

Myrrh inhibits LPS-induced inflammatory response and protects from cecal ligation and puncture-induced sepsis.

Kim M, Bae G, Park K, Koo B, Kim B, Lee H Evid Based Complement Alternat Med. 2011; 2012:278718.

PMID: 21826187 PMC: 3151005. DOI: 10.1155/2012/278718.

References

Berrigan M, STRUCK R, Gurtoo H . Lipid peroxidation induced by cyclophosphamide. Cancer Biochem Biophys. 1987; 9(3):265-70. View

Schmid W . The micronucleus test. Mutat Res. 1975; 31(1):9-15. DOI: 10.1016/0165-1161(75)90058-8. View

Oleinik A . [Effect of cyclophosphane on lipid peroxidation]. Vopr Onkol. 1985; 31(7):97-101. View

Rozengurt E, Mendoza S . Monovalent ion fluxes and the control of cell proliferation in cultured fibroblasts. Ann N Y Acad Sci. 1980; 339:175-90. DOI: 10.1111/j.1749-6632.1980.tb15977.x. View

Smith J, Rozengurt E . Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc Natl Acad Sci U S A. 1978; 75(11):5560-4. PMC: 393006. DOI: 10.1073/pnas.75.11.5560. View

Thompson D, Constantin-Teodosiu D, Moldeus P . Metabolism and cytotoxicity of eugenol in isolated rat hepatocytes. Chem Biol Interact. 1991; 77(2):137-47. DOI: 10.1016/0009-2797(91)90069-j. View

Alldrick A, Lake B, Rowland I . Modification of in vivo heterocyclic amine genotoxicity by dietary flavonoids. Mutagenesis. 1989; 4(5):365-70. DOI: 10.1093/mutage/4.5.365. View

Fong K, MCCAY P, Poyer J, Keele B, Misra H . Evidence that peroxidation of lysosomal membranes is initiated by hydroxyl free radicals produced during flavin enzyme activity. J Biol Chem. 1973; 248(22):7792-7. View

Al-Bekairi A, Qureshi S, Chaudhry M, Shah A . Uric acid as an inhibitor of cyclophosphamide-induced micronuclei in mice. Mutat Res. 1991; 262(2):115-8. DOI: 10.1016/0165-7992(91)90116-l. View

10.

Patel J . Stimulation of cyclophosphamide-induced pulmonary microsomal lipid peroxidation by oxygen. Toxicology. 1987; 45(1):79-91. DOI: 10.1016/0300-483x(87)90116-8. View

11.

Villereal M . Sodium fluxes in human fibroblasts: effect of serum, Ca+2, and amiloride. J Cell Physiol. 1981; 107(3):359-69. DOI: 10.1002/jcp.1041070307. View

12.

Whitehouse R, Prasad A, Rabbani P, Cossack Z . Zinc in plasma, neutrophils, lymphocytes, and erythrocytes as determined by flameless atomic absorption spectrophotometry. Clin Chem. 1982; 28(3):475-80. View

13.

Sparks R, Pool T, Smith N, Cameron I . Effects of amiloride on tumor growth and intracellular element content of tumor cells in vivo. Cancer Res. 1983; 43(1):73-7. View

14.

Oleinik A . [Effect of cyclophosphane on bile formation and lipid peroxidation in the liver]. Farmakol Toksikol. 1986; 49(4):51-4. View

15.

Tariq M, Ageel A, Al-Yahya M, Mossa J, Al-Said M, Parmar N . Anti-inflammatory activity of Commiphora molmol. Agents Actions. 1986; 17(3-4):381-2. DOI: 10.1007/BF01982655. View

16.

Borek C . Oncogenes, hormones, and free-radical processes in malignant transformation in vitro. Ann N Y Acad Sci. 1988; 551:95-101; discussion 102. DOI: 10.1111/j.1749-6632.1988.tb22323.x. View

17.

Al-Bekairi A, Qureshi S, Ahmed M, Qazi N, Khan Z, Shah A . Effect of Caralluma tuberculata on the cytological and biochemical changes induced by cyclophosphamide in mice. Food Chem Toxicol. 1992; 30(8):719-22. DOI: 10.1016/0278-6915(92)90169-l. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Koch K, Leffert H . Increased sodium ion influx is necessary to initiate rat hepatocyte proliferation. Cell. 1979; 18(1):153-63. DOI: 10.1016/0092-8674(79)90364-7. View

20.

KALTENBACH J, KALTENBACH M, Lyons W . Nigrosin as a dye for differentiating live and dead ascites cells. Exp Cell Res. 1958; 15(1):112-7. DOI: 10.1016/0014-4827(58)90067-3. View