Pentoxifylline: a Potent Inhibitor of IL-2 and IFN-gamma Biosynthesis and BCG-induced Cytotoxicity

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1993 Sep 1

PMID 8244458

Citations 16

Authors

A Thanhauser

N Reiling

A Bohle

K M Toellner

M Duchrow

D Scheel

C Schluter

M Ernst

H D Flad

A J Ulmer

Affiliations

Soon will be listed here.

Abstract

In the present study we investigated the influence of pentoxifylline (POF) on bacillus Calmette-Guérin (BCG)- and phytohaemagglutinin (PHA)-induced DNA synthesis and cytokine release, and BCG-induced cytotoxicity of human peripheral blood mononuclear cells (PBMC). DNA synthesis of PBMC stimulated with either BCG or PHA was inhibited by POF. We also demonstrated that the addition of POF led to a POF dose-dependent decrease of the release of the cytokines interleukin (IL)-2, interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha). The release of IL-6 remained unaffected. With respect to the inhibition of BCG-induced IL-2 and IFN-gamma release POF is active at the transcriptional (mRNA) level, as found by polymerase chain reaction (PCR). However, PHA-induced mRNA expression of these lymphokines is not affected by POF. Thus, the existence of a post-transcriptional regulation of PHA-induced cytokine release by POF can be assumed. The observed inhibition of cytokine release is correlated with a potent inhibitory effect of POF on BCG-induced cytotoxicity against bladder tumour cell lines. This effect is reversible.

Citing Articles

Treatment of Poor Sperm Quality and Erectile Dysfunction With Oral Pentoxifylline: A Systematic Review.

Lu Y, Su H, Zhang J, Wang Y, Li H Front Pharmacol. 2022; 12:789787.

PMID: 35095501 PMC: 8790020. DOI: 10.3389/fphar.2021.789787.

Effects of pentoxifylline on whole blood IL-2 and IFN-gamma gene expression in normal dogs.

Kummari E, Gibbs A, Riggs C, Fellman C, Stokes J, Thomason J Vet Med Sci. 2019; 6(1):19-24.

PMID: 31621209 PMC: 7036308. DOI: 10.1002/vms3.204.

Effect of pentoxifylline on semen parameters, reproductive hormones, and seminal plasma antioxidant capacity in men with idiopathic infertility: a randomized double-blind placebo-controlled study.

Safarinejad M Int Urol Nephrol. 2010; 43(2):315-28.

PMID: 20820916 DOI: 10.1007/s11255-010-9826-4.

Steroid-sparing effects of pentoxifylline in pulmonary sarcoidosis.

Park M, Fontana Jr , Babaali H, Gilbert-McClain L, Stylianou M, Joo J Sarcoidosis Vasc Diffuse Lung Dis. 2010; 26(2):121-31.

PMID: 20560292 PMC: 2946799.

Pentoxifylline inhibits the synthesis and IFN-gamma-inducing activity of IL-18.

Samardzic T, Jankovic V, Stosic-Grujicic S, Popadic D, Trajkovic V Clin Exp Immunol. 2001; 124(2):274-81.

PMID: 11422205 PMC: 1906051. DOI: 10.1046/j.1365-2249.2001.01546.x.

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