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Heat-induced Requirements for Sucrose or Magnesium for Expression of Heat Resistance in Bacillus Cereus Forespores

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1976 Aug 1
PMID 823870
Citations 2
Authors
F F Busta
E Baillie
W G Murrell
Affiliations
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Abstract

The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect forespore germination or outgrowth, but injured cells in the absence of sucrose or Mg2+ were not capable of cell division. The heat-induced sublethal damage apparently affected the forspore component(s) that could be repaired or was capable of normal function in the presence of added Mg2+ or sucrose.

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Osmotically induced increase in thermal resistance of heat-sensitive, dipicolinic acid-less spores of Bacillus cereus Ht-8.

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