Fractionation of RNA from Tetahymena by Affinity Chromatography on Poly-U-Sepharose

Overview

Journal J Mol Evol

Specialty Biochemistry

Date 1976 Aug 3

PMID 823341

Citations 2

Authors

A Ron

O Horovitz

I Sarov

Affiliations

Soon will be listed here.

Abstract

Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion. Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.

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