Expression of Pregnancy-specific Beta 1-glycoprotein Genes in Hematopoietic Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1993 May 26

PMID 8232246

Citations 5

Authors

S M Wu

L S Bazar

M L COHN

R A Cahill

W Y Chan

Affiliations

Soon will be listed here.

Abstract

The presence of PSG in blood cells has been demonstrated by immunohistochemical staining. However, the origin of those proteins is not known. This report examines the expression of the PSG genes in different types of freshly isolated blood cells. RNA isolated from bone marrow and peripheral blood cells of healthy individuals was analyzed for PSG transcripts by reverse transcriptase-polymerase chain reaction using synthetic oligonucleotide primers specific for the PSG genes. The level of expression of the PSG genes in different types of cells exhibited significant individual variation. Trace amounts of PSG transcripts could be detected in polymorphonuclear cells (PMN), monocytes and B lymphocytes while T lymphocytes always contained the highest level of transcript. The expression of PSG genes in the blood cells apparently was not affected by the method of isolation nor by overnight culturing of these cells except in the case when lymphocytes were separated by rosetting with sheep red blood cells. All reported PSG transcripts were detected in blood cells. Both type I and type II transcripts of the PSG genes were detected in blood cells with the exception of type II transcript of PSG5 and PSG11 which were only found in the placenta. Tissue specificity in the expression or alternative splicing of some of the PSG family members was implicated.

Citing Articles

PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins.

Camolotto S, Racca A, Ridano M, Genti-Raimondi S, Panzetta-Dutari G PLoS One. 2013; 8(2):e55992.

PMID: 23418492 PMC: 3572148. DOI: 10.1371/journal.pone.0055992.

Transcription of genes encoding pregnancy-specific glycoproteins is regulated by negative promoter-selective elements.

Panzetta-Dutari G, Koritschoner N, Bocco J, Nores R, Dumur C, Patrito L Biochem J. 2000; 350 Pt 2:511-9.

PMID: 10947966 PMC: 1221279.

Characterization and cellular localization of PSG in rat testis.

Blomberg L, Wu S, Dirami G, Dym M, Chou J, Chan W Mol Cell Biochem. 1998; 177(1-2):229-37.

PMID: 9450667 DOI: 10.1023/a:1006811305616.

Evolutionary analysis of the multigene pregnancy-specific beta 1-glycoprotein family: separation of historical and nonhistorical signals.

McLenachan P, Lockhart P, Faber H, Mansfield B J Mol Evol. 1996; 42(2):273-80.

PMID: 8919879 DOI: 10.1007/BF02198854.

Genetic heterogeneity of constitutively activating mutations of the human luteinizing hormone receptor in familial male-limited precocious puberty.

Laue L, Chan W, Hsueh A, Kudo M, Hsu S, Wu S Proc Natl Acad Sci U S A. 1995; 92(6):1906-10.

PMID: 7892197 PMC: 42391. DOI: 10.1073/pnas.92.6.1906.

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