The DNA-binding Domain of Simian Virus 40 Tumor Antigen Has Multiple Functions

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1993 Dec 1

PMID 8230479

Citations 38

Authors

R Upson

W Young

T Melendy

B Stillman

D T Simmons

Affiliations

Soon will be listed here.

Abstract

The DNA-binding domain of simian virus 40 tumor antigen has been previously shown to participate in a number of different activities. Besides being involved in binding to sequences at the viral replication origin, this domain appears to be required for nonspecific DNA binding, for structurally distorting origin DNA (melting and untwisting), and possibly for oligomerization of the protein into hexamers and double hexamers. We now provide evidence that it also takes part in unwinding origin DNA sequences, contributes a function specifically related to in vivo DNA replication, and perhaps supports the assembly of the virus or release of the virus from the cell. This 100-amino-acid domain appears to be an excellent model system for studying how a small region of a protein could have a number of distinct activities.

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References

Maulbecker C, MOHR I, Gluzman Y, Bartholomew J, Botchan M . A deletion in the simian virus 40 large T antigen impairs lytic replication in monkey cells in vivo but enhances DNA replication in vitro: new complementation function of T antigen. J Virol. 1992; 66(4):2195-207. PMC: 289012. DOI: 10.1128/JVI.66.4.2195-2207.1992. View

Parsons R, Stenger J, Ray S, Welker R, Anderson M, Tegtmeyer P . Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin. J Virol. 1991; 65(6):2798-806. PMC: 240896. DOI: 10.1128/JVI.65.6.2798-2806.1991. View

SOMPAYRAC L, Danna K . Efficient infection of monkey cells with DNA of simian virus 40. Proc Natl Acad Sci U S A. 1981; 78(12):7575-8. PMC: 349311. DOI: 10.1073/pnas.78.12.7575. View

DeLucia A, Lewton B, Tjian R, Tegtmeyer P . Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication. J Virol. 1983; 46(1):143-50. PMC: 255102. DOI: 10.1128/JVI.46.1.143-150.1983. View

Gralla J . Rapid "footprinting" on supercoiled DNA. Proc Natl Acad Sci U S A. 1985; 82(10):3078-81. PMC: 397717. DOI: 10.1073/pnas.82.10.3078. View

Wobbe C, Dean F, Weissbach L, Hurwitz J . In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site. Proc Natl Acad Sci U S A. 1985; 82(17):5710-4. PMC: 390621. DOI: 10.1073/pnas.82.17.5710. View

Simanis V, Lane D . An immunoaffinity purification procedure for SV40 large T antigen. Virology. 1985; 144(1):88-100. DOI: 10.1016/0042-6822(85)90308-3. View

Stillman B, Gerard R, Guggenheimer R, Gluzman Y . T antigen and template requirements for SV40 DNA replication in vitro. EMBO J. 1985; 4(11):2933-9. PMC: 554601. DOI: 10.1002/j.1460-2075.1985.tb04026.x. View

Simmons D . DNA-binding region of the simian virus 40 tumor antigen. J Virol. 1986; 57(3):776-85. PMC: 252805. DOI: 10.1128/JVI.57.3.776-785.1986. View

10.

Dean F, Bullock P, Murakami Y, Wobbe C, Weissbach L, Hurwitz J . Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication. Proc Natl Acad Sci U S A. 1987; 84(1):16-20. PMC: 304132. DOI: 10.1073/pnas.84.1.16. View

11.

Strauss M, Argani P, Mohr I, Gluzman Y . Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen. J Virol. 1987; 61(10):3326-30. PMC: 255919. DOI: 10.1128/JVI.61.10.3326-3330.1987. View

12.

Goetz G, Dean F, Hurwitz J, Matson S . The unwinding of duplex regions in DNA by the simian virus 40 large tumor antigen-associated DNA helicase activity. J Biol Chem. 1988; 263(1):383-92. View

13.

Dean F, DODSON M, Echols H, Hurwitz J . ATP-dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin. Proc Natl Acad Sci U S A. 1987; 84(24):8981-5. PMC: 299675. DOI: 10.1073/pnas.84.24.8981. View

14.

Prelich G, Stillman B . Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNA. Cell. 1988; 53(1):117-26. DOI: 10.1016/0092-8674(88)90493-x. View

15.

Simmons D . Geometry of the simian virus 40 large tumor antigen-DNA complex as probed by protease digestion. Proc Natl Acad Sci U S A. 1988; 85(7):2086-90. PMC: 279933. DOI: 10.1073/pnas.85.7.2086. View

16.

Arthur A, Hoss A, Fanning E . Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids. J Virol. 1988; 62(6):1999-2006. PMC: 253284. DOI: 10.1128/JVI.62.6.1999-2006.1988. View

17.

Borowiec J, Hurwitz J . Localized melting and structural changes in the SV40 origin of replication induced by T-antigen. EMBO J. 1988; 7(10):3149-58. PMC: 454705. DOI: 10.1002/j.1460-2075.1988.tb03182.x. View

18.

Loeber G, Parsons R, Tegtmeyer P . The zinc finger region of simian virus 40 large T antigen. J Virol. 1989; 63(1):94-100. PMC: 247661. DOI: 10.1128/JVI.63.1.94-100.1989. View

19.

Mastrangelo I, HOUGH P, Wall J, DODSON M, Dean F, Hurwitz J . ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication. Nature. 1989; 338(6217):658-62. DOI: 10.1038/338658a0. View

20.

Bullock P, Seo Y, Hurwitz J . Initiation of simian virus 40 DNA replication in vitro: pulse-chase experiments identify the first labeled species as topologically unwound. Proc Natl Acad Sci U S A. 1989; 86(11):3944-8. PMC: 287364. DOI: 10.1073/pnas.86.11.3944. View