K252a: a New Blocker of the Cell-cycle at G1 Phase in a Human Hepatoma Cell Line

Overview

Journal Experientia

Specialty Science

Date 1993 Oct 15

PMID 8224104

Authors

T Nakayama

Y Hashimoto

Y Kaneko

M Yoshida

T Beppu

K Ohmi

S Yamashita

Y Nonomura

K Kurokawa

Affiliations

Soon will be listed here.

Abstract

The administration of 200 nM K252a to HuH7 suppressed the proliferation of the cells almost completely. The uptake of [3H]thymidine was inhibited, and flow cytometry revealed only one peak at 2C on day 3 after treatment with 100 nM K252a. The expression of proto-oncogene c-myc was not reduced. Despite the blockage at G1, both the size of the cells and the amount of cell protein had increased by 4 times by day 3 after treatment with K252a, while the cells secreted albumin and alpha-fetoprotein into the medium as usual. These results show that K252a can increase the cell size of HuH7 without losing its function by blocking the cell cycle at G1 phase.

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