The Plant Mitochondrial Open Reading Frame Orf221 Encodes a Membrane-bound Protein

Overview

Journal Plant Mol Biol

Date 1993 Oct 1

PMID 8219065

Citations 6

Authors

L M Prioli

J Huang

C S Levings 3rd

Affiliations

Soon will be listed here.

Abstract

We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.

Citing Articles

Interaction of nuclear and mitochondrial genomes in the alteration of maize mitochondrial orf221 transcripts.

Rocheford T, Pring D Theor Appl Genet. 2013; 89(7-8):951-8.

PMID: 24178109 DOI: 10.1007/BF00224523.

Interactions of mitochondrial and nuclear genes that affect male gametophyte development.

Hanson M, Bentolila S Plant Cell. 2004; 16 Suppl:S154-69.

PMID: 15131248 PMC: 2643387. DOI: 10.1105/tpc.015966.

The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b.

Burger G, Lang B, Braun H, Marx S Nucleic Acids Res. 2003; 31(9):2353-60.

PMID: 12711680 PMC: 154212. DOI: 10.1093/nar/gkg326.

Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green algae.

Burger G, Gray M, Lang B Plant Cell. 1999; 11(9):1675-94.

PMID: 10488235 PMC: 144311. DOI: 10.1105/tpc.11.9.1675.

Genome structure and gene content in protist mitochondrial DNAs.

Gray M, Lang B, Cedergren R, Golding G, Lemieux C, Sankoff D Nucleic Acids Res. 1998; 26(4):865-78.

PMID: 9461442 PMC: 147373. DOI: 10.1093/nar/26.4.865.

References

Korth K, Kaspi C, Siedow J, Levings 3rd C . URF13, a maize mitochondrial pore-forming protein, is oligomeric and has a mixed orientation in Escherichia coli plasma membranes. Proc Natl Acad Sci U S A. 1991; 88(23):10865-9. PMC: 53032. DOI: 10.1073/pnas.88.23.10865. View

Brandt P, Sunkel S, Unseld M, Brennicke A, Knoop V . The nad4L gene is encoded between exon c of nad5 and orf25 in the Arabidopsis mitochondrial genome. Mol Gen Genet. 1992; 236(1):33-8. DOI: 10.1007/BF00279640. View

Neuburger M, Journet E, Bligny R, Carde J, Douce R . Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys. 1982; 217(1):312-23. DOI: 10.1016/0003-9861(82)90507-0. View

Dewey R, Timothy D, Levings C . A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize. Proc Natl Acad Sci U S A. 1987; 84(15):5374-8. PMC: 298858. DOI: 10.1073/pnas.84.15.5374. View

Bonen L, Bird S, Belanger L . Characterization of the wheat mitochondrial orf25 gene. Plant Mol Biol. 1990; 15(5):793-5. DOI: 10.1007/BF00016131. View

Anderson D, Blobel G . Immunoprecipitation of proteins from cell-free translations. Methods Enzymol. 1983; 96:111-20. DOI: 10.1016/s0076-6879(83)96012-3. View

Levings 3rd C . The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science. 1990; 250(4983):942-7. DOI: 10.1126/science.250.4983.942. View

Remaut E, Stanssens P, Fiers W . Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene. 1981; 15(1):81-93. DOI: 10.1016/0378-1119(81)90106-2. View

Dawson A, Jones V, Leaver C . The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J. 1984; 3(9):2107-13. PMC: 557650. DOI: 10.1002/j.1460-2075.1984.tb02098.x. View

10.

Wise R, Pring D, Gengenbach B . Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame. Proc Natl Acad Sci U S A. 1987; 84(9):2858-62. PMC: 304759. DOI: 10.1073/pnas.84.9.2858. View

11.

Liu A, Narayanan K, Andre C, Kaleikau E, Walbot V . Co-transcription of orf25 and coxIII in rice mitochondria. Curr Genet. 1992; 21(6):507-13. DOI: 10.1007/BF00351661. View

12.

Ward G, Levings 3rd C . The protein-encoding gene T-urf13 is not edited in maize mitochondria. Plant Mol Biol. 1991; 17(5):1083-8. DOI: 10.1007/BF00037148. View

13.

Dewey R, Levings 3rd C, Timothy D . Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986; 44(3):439-49. DOI: 10.1016/0092-8674(86)90465-4. View

14.

Wang J, Barth J, Abbott A . Higher plant mitochondrial DNA expression : 1. Variant expression of the plant mitochondrial open reading frame, ORF25, in B37N and B73N maize lines. Theor Appl Genet. 2013; 82(6):765-70. DOI: 10.1007/BF00227323. View

15.

Fujiki Y, Fowler S, Shio H, Hubbard A, Lazarow P . Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes. J Cell Biol. 1982; 93(1):103-10. PMC: 2112093. DOI: 10.1083/jcb.93.1.103. View

16.

Dewey R, Siedow J, Timothy D, Levings 3rd C . A 13-kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin. Science. 1988; 239(4837):293-5. DOI: 10.1126/science.3276005. View

17.

Stamper S, Dewey R, Bland M, Levings 3rd C . Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria. Curr Genet. 1987; 12(6):457-63. DOI: 10.1007/BF00434824. View

18.

Vieira J, Messing J . Production of single-stranded plasmid DNA. Methods Enzymol. 1987; 153:3-11. DOI: 10.1016/0076-6879(87)53044-0. View

19.

Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N . Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. A primitive form of plant mitochondrial genome. J Mol Biol. 1992; 223(1):1-7. DOI: 10.1016/0022-2836(92)90708-r. View

20.

Leaver C, Hack E, Forde B . Protein synthesis by isolated plant mitochondria. Methods Enzymol. 1983; 97:476-84. DOI: 10.1016/0076-6879(83)97156-2. View