Comparison and Genomic Sizing of Escherichia Coli O157:H7 Isolates by Pulsed-field Gel Electrophoresis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1993 Sep 1

PMID 8215383

Citations 27

Authors

K D Harsono

C W Kaspar

J. B. Luchansky

Affiliations

Soon will be listed here.

Abstract

Genomic DNAs of Escherichia coli O157:H7 strains isolated from patients and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distinct genomic profiles, respectively, for the 22 strains analyzed, indicating that this technique may find application for epidemiologic studies. Summation of XbaI fragments from five E. coli O157:H7 strains estimated the genomic length at ca. 4.7 Mb.

Citing Articles

A sugar utilization phenotype contributes to the formation of genetic exchange communities in lactic acid bacteria.

Takenaka S, Kawashima T, Arita M FEMS Microbiol Lett. 2021; 368(17).

PMID: 34468734 PMC: 8440127. DOI: 10.1093/femsle/fnab117.

A review on detection methods used for foodborne pathogens.

Priyanka B, Patil R, Dwarakanath S Indian J Med Res. 2017; 144(3):327-338.

PMID: 28139531 PMC: 5320838. DOI: 10.4103/0971-5916.198677.

Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan.

Kusumoto M, Hikoda Y, Fujii Y, Murata M, Miyoshi H, Ogura Y J Clin Microbiol. 2016; 54(4):1074-81.

PMID: 26865687 PMC: 4809958. DOI: 10.1128/JCM.03141-15.

Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection.

Kudva I, Smole S, Griffin R, Garren J, Kalia N, Murray M Open Microbiol J. 2013; 7:123-9.

PMID: 24278089 PMC: 3837366. DOI: 10.2174/1874285801307010123.

Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates.

Kudva I, Davis M, Griffin R, Garren J, Murray M, John M Int J Microbiol. 2012; 2012:140105.

PMID: 23049559 PMC: 3461292. DOI: 10.1155/2012/140105.

References

Szabo E, Desmarchelier P . A comparative study of clinical and food isolates of Listeria monocytogenes and related species. Epidemiol Infect. 1990; 105(2):245-54. PMC: 2271887. DOI: 10.1017/s0950268800047853. View

Prevost G, Jaulhac B, Piemont Y . DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol. 1992; 30(4):967-73. PMC: 265195. DOI: 10.1128/jcm.30.4.967-973.1992. View

Padhye N, Doyle M . Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food. Appl Environ Microbiol. 1991; 57(9):2693-8. PMC: 183642. DOI: 10.1128/aem.57.9.2693-2698.1991. View

Johnson J, Moseley S, COYLE M, Stamm W . Success of DNA fingerprinting after failure of biotyping, antimicrobial susceptibility testing, and plasmid analysis to reveal clonality of multiple blood and urine isolates from a patient with Escherichia coli urosepsis. Diagn Microbiol Infect Dis. 1992; 15(5):399-405. DOI: 10.1016/0732-8893(92)90080-d. View

Arbeit R, Arthur M, Dunn R, Kim C, Selander R, Goldstein R . Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. J Infect Dis. 1990; 161(2):230-5. DOI: 10.1093/infdis/161.2.230. View

Bohm H, Karch H . DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1992; 30(8):2169-72. PMC: 265464. DOI: 10.1128/jcm.30.8.2169-2172.1992. View

Baloga A, Harlander S . Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys. Appl Environ Microbiol. 1991; 57(8):2324-31. PMC: 183571. DOI: 10.1128/aem.57.8.2324-2331.1991. View

Smith C, Econome J, Schutt A, Klco S, Cantor C . A physical map of the Escherichia coli K12 genome. Science. 1987; 236(4807):1448-53. DOI: 10.1126/science.3296194. View

Ostroff S, Tarr P, Neill M, Lewis J, Kobayashi J . Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J Infect Dis. 1989; 160(6):994-8. DOI: 10.1093/infdis/160.6.994. View

10.

Canard B, Cole S . Genome organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci U S A. 1989; 86(17):6676-80. PMC: 297908. DOI: 10.1073/pnas.86.17.6676. View

11.

Griffin P, Tauxe R . The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991; 13:60-98. DOI: 10.1093/oxfordjournals.epirev.a036079. View

12.

J. B. Luchansky , Glass K, Harsono K, Degnan A, Faith N, Cauvin B . Genomic analysis of Pediococcus starter cultures used to control Listeria monocytogenes in turkey summer sausage. Appl Environ Microbiol. 1992; 58(9):3053-9. PMC: 183047. DOI: 10.1128/aem.58.9.3053-3059.1992. View

13.

Perkins J, Heath J, Sharma B, Weinstock G . SfiI genomic cleavage map of Escherichia coli K-12 strain MG1655. Nucleic Acids Res. 1992; 20(5):1129-37. PMC: 312102. DOI: 10.1093/nar/20.5.1129. View

14.

Gordillo M, Reeve G, Pappas J, Mathewson J, DuPont H, Murray B . Molecular characterization of strains of enteroinvasive Escherichia coli O143, including isolates from a large outbreak in Houston, Texas. J Clin Microbiol. 1992; 30(4):889-93. PMC: 265180. DOI: 10.1128/jcm.30.4.889-893.1992. View

15.

Doyle M . Escherichia coli O157:H7 and its significance in foods. Int J Food Microbiol. 1991; 12(4):289-301. DOI: 10.1016/0168-1605(91)90143-d. View

16.

Howard P, Harsono K, J. B. Luchansky . Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis. Appl Environ Microbiol. 1992; 58(2):709-12. PMC: 195308. DOI: 10.1128/aem.58.2.709-712.1992. View

17.

Scherer S, Stevens D . A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci U S A. 1988; 85(5):1452-6. PMC: 279789. DOI: 10.1073/pnas.85.5.1452. View

18.

Ott M, Bender L, Blum G, Schmittroth M, Achtman M, Tschape H . Virulence patterns and long-range genetic mapping of extraintestinal Escherichia coli K1, K5, and K100 isolates: use of pulsed-field gel electrophoresis. Infect Immun. 1991; 59(8):2664-72. PMC: 258071. DOI: 10.1128/iai.59.8.2664-2672.1991. View

19.

Ratnam S, March S, Ahmed R, Bezanson G, Kasatiya S . Characterization of Escherichia coli serotype O157:H7. J Clin Microbiol. 1988; 26(10):2006-12. PMC: 266806. DOI: 10.1128/jcm.26.10.2006-2012.1988. View

20.

Tanskanen E, Tulloch D, Hillier A, Davidson B . Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification. Appl Environ Microbiol. 1990; 56(10):3105-11. PMC: 184906. DOI: 10.1128/aem.56.10.3105-3111.1990. View