Evaluation of Diamine Oxidase Activity (DAO) in the Rat Intestinal Mucosa by Measuring Expired 14CO2 After Oral Administration of 14C-putrescine

Overview

Journal J Gastroenterol

Specialty Gastroenterology

Date 1994 Feb 1

PMID 8199691

Authors

T Bamba

A Sasaki

S Hosoda

Affiliations

Soon will be listed here.

Abstract

This study was performed to investigate whether mucosal diamine oxidase activity could be assessed by measuring expired 14CO2 after oral administration of 14C-putrescine. Immediately after giving 5 mu Ci of 14C-putrescine, the 14CO2 was collected at 1-h intervals for 8 h into a vial containing 1 ml of 10 mM hyamine hydroxide, 2 ml of ethanol, and an appropriate amount of phenolphthalein dye. The expired 14CO2 caused the color to disappear. The amount of 14CO2, determined by scintillation counting, reached a maximum 1 h after 14C-putrescine administration, and gradually decreased thereafter. A positive correlation between the mucosal diamine oxidase activity and the maximal expired 14CO2 value was obtained. There is no doubt that this test can be used to easily detect mucosal DAO activity and avoid the necessity of mucosal biopsy.

References

Pegg A, McCann P . Polyamine metabolism and function. Am J Physiol. 1982; 243(5):C212-21. DOI: 10.1152/ajpcell.1982.243.5.C212. View

Tamura H, Horiike K, Fukuda H, Watanabe T . Kinetic studies on the inhibition mechanism of diamine oxidase from porcine kidney by aminoguanidine. J Biochem. 1989; 105(2):299-306. DOI: 10.1093/oxfordjournals.jbchem.a122657. View

Tryding N, WILLERT B . Determination of plasma diamine oxidase (histaminase) in clinical practice. A comparison between a biological method and a radiochemical micromethod. Scand J Clin Lab Invest. 1968; 22(1):29-32. DOI: 10.3109/00365516809160732. View

Baylin S, Stevens S, Shakir K . Association of diamine oxidase and ornithine decarboxylase with maturing cells in rapidly proliferating epithelium. Biochim Biophys Acta. 1978; 541(3):415-9. DOI: 10.1016/0304-4165(78)90200-3. View

Roberts E, Bregoff H . Transamination of gamma-aminobutyric acid and beta-alanine in brain and liver. J Biol Chem. 1953; 201(1):393-8. View

Shakir K, Margolis S, Baylin S . Localization of histamine (diamine oxidase) in rat small intestinal mucosa: site of release by heparin. Biochem Pharmacol. 1977; 26(24):2343-7. DOI: 10.1016/0006-2952(77)90438-5. View

Rokkas T, Vaja S, Taylor P, Murphy G, Dowling R . Is the intestine the sole source of heparin-stimulated plasma diamine oxidase? Acute effects of jejunectomy, ileectomy and total enterectomy. Digestion. 1990; 46 Suppl 2:439-46. DOI: 10.1159/000200419. View

Rokkas T, Vaja S, Taylor P, Murphy G, Dowling R . Effect of intestinal diamine oxidase (DAO) depletion by heparin on mucosal polyamine metabolism. Digestion. 1990; 46 Suppl 2:378-82. DOI: 10.1159/000200411. View

Buts J, Theys S, de Keyser N, Dive C . Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats. Correlation of DAO activity with mucosal mass parameters. Dig Dis Sci. 1989; 34(9):1393-8. DOI: 10.1007/BF01538075. View

10.

Kind P, King E . Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954; 7(4):322-6. PMC: 1023845. DOI: 10.1136/jcp.7.4.322. View

11.

Kusche J, Izbicki J, Mennigen R, Curt A, Parkin J . The effect of experimental carcinogenesis on intestinal diamine oxidase, a polyamine deaminating enzyme. Cancer Detect Prev. 1986; 9(1-2):17-29. View

12.

Luk G, Bayless T, Baylin S . Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat. J Clin Invest. 1983; 71(5):1308-15. PMC: 436992. DOI: 10.1172/jci110881. View

13.

Seiler N . Putrescine catabolism in mammalian brain. Biochem J. 1974; 144(1):29-35. PMC: 1168460. DOI: 10.1042/bj1440029. View

14.

dAgostino L, Ciacci C, Daniele B, Barone M, Sollazzo R, Mazzacca G . Postheparin plasma diamine oxidase in subjects with small bowel mucosal atrophy. Dig Dis Sci. 1987; 32(3):313-7. DOI: 10.1007/BF01297059. View

15.

Mennigen R, Kusche J, Streffer C, Krakamp B . Diamine oxidase activities in the large bowel mucosa of ulcerative colitis patients. Agents Actions. 1990; 30(1-2):264-6. DOI: 10.1007/BF01969056. View

16.

Rokkas T, Vaja S, Murphy G, Dowling R . Postheparin plasma diamine oxidase in health and intestinal disease. Gastroenterology. 1990; 98(6):1493-501. DOI: 10.1016/0016-5085(90)91081-g. View

17.

Fogel W, Bieganski T, Wozniak J, Maslinski C . Interference of aldehyde metabolizing enzymes with diamine oxidase/histaminase/activity as determined by 14C putrescine method. Biochem Pharmacol. 1978; 27(8):1159-62. DOI: 10.1016/0006-2952(78)90445-8. View

18.

Hosoda N, Nishi M, Nakagawa M, Hiramatsu Y, Hioki K, Yamamoto M . Structural and functional alterations in the gut of parenterally or enterally fed rats. J Surg Res. 1989; 47(2):129-33. DOI: 10.1016/0022-4804(89)90076-0. View

19.

Luk G, Bayless T, Baylin S . Diamine oxidase (histaminase). A circulating marker for rat intestinal mucosal maturation and integrity. J Clin Invest. 1980; 66(1):66-70. PMC: 371506. DOI: 10.1172/JCI109836. View

20.

BESSMAN S, Rossen J, LAYNE E . Gamma-Aminobutyric acid-glutamic acid transamination in brain. J Biol Chem. 1953; 201(1):385-91. View