» Articles » PMID: 817632

Carbohydrate Analysis of Concanavalin A-reactive and Concanavalin A-nonreactive Mycobacterial Polysaccharides

Overview
Specialty Pulmonary Medicine
Date 1976 May 1
PMID 817632
Citations 7
Authors
Affiliations
Soon will be listed here.
Abstract

Concanavalin A-nonreactive polysaccharide and 2 concanavalin A-reactive polysaccharides of differing concanavalin A affinities that had been purified from culture filtrates of Mycobacterium tuberculosis were subjected to carbohydrate analysis by gas and gas-liquid chromatography. Concanavalin A-nonreactive polysaccharide was found to be D-arabinogalactan, and the concanavalin A-reactive polysaccharide of highest affinity was found to be D-arabinomannan. The lower affinity concanavalin A-reactive polysaccharide was rich in arabinose and probably represented a mixture, with more than one polysaccharide present. Glucan was found to be a significant contaminant of all 3 polysaccharides.

Citing Articles

Isolation and partial characterization of glycolipid fractions from Mycobacterium avium serovar 2 (Mycobacterium paratuberculosis 18) that inhibit activated macrophages.

Hines 2nd M, Jaynes J, Barker S, Newton J, Enright F, Snider 3rd T Infect Immun. 1993; 61(1):1-7.

PMID: 8418031 PMC: 302680. DOI: 10.1128/iai.61.1.1-7.1993.


Suppression of lymphocyte responses by tuberculous plasma and mycobacterial arabinogalactan. Monocyte dependence and indomethacin reversibility.

Kleinhenz M, Ellner J, Spagnuolo P, Daniel T J Clin Invest. 1981; 68(1):153-62.

PMID: 6972952 PMC: 370783. DOI: 10.1172/jci110231.


Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.

Daniel T, Gonchoroff N, Katzmann J, Olds G Infect Immun. 1984; 45(1):52-5.

PMID: 6376360 PMC: 263262. DOI: 10.1128/iai.45.1.52-55.1984.


Lipoarabinomannan and lipid-free arabinomannan antigens of Mycobacterium paratuberculosis.

Sugden E, Samagh B, Bundle D, Duncan J Infect Immun. 1987; 55(3):762-70.

PMID: 3818096 PMC: 260408. DOI: 10.1128/iai.55.3.762-770.1987.


Demonstration of a shared epitope among mycobacterial antigens using a monoclonal antibody.

Daniel T, Olds G Clin Exp Immunol. 1985; 60(2):249-58.

PMID: 2408799 PMC: 1577034.