Carbohydrate Analysis of Concanavalin A-reactive and Concanavalin A-nonreactive Mycobacterial Polysaccharides

Overview

Journal Am Rev Respir Dis

Specialty Pulmonary Medicine

Date 1976 May 1

PMID 817632

Citations 7

Authors

T M Daniel

A Misaki

Affiliations

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Abstract

Concanavalin A-nonreactive polysaccharide and 2 concanavalin A-reactive polysaccharides of differing concanavalin A affinities that had been purified from culture filtrates of Mycobacterium tuberculosis were subjected to carbohydrate analysis by gas and gas-liquid chromatography. Concanavalin A-nonreactive polysaccharide was found to be D-arabinogalactan, and the concanavalin A-reactive polysaccharide of highest affinity was found to be D-arabinomannan. The lower affinity concanavalin A-reactive polysaccharide was rich in arabinose and probably represented a mixture, with more than one polysaccharide present. Glucan was found to be a significant contaminant of all 3 polysaccharides.

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