Lactobacillus Acidophilus LA 1 Binds to Cultured Human Intestinal Cell Lines and Inhibits Cell Attachment and Cell Invasion by Enterovirulent Bacteria

Overview

Journal Gut

Specialty Gastroenterology

Date 1994 Apr 1

PMID 8174985

Citations 165

Authors

M F Bernet

D Brassart

J R Neeser

A L Servin

Affiliations

Soon will be listed here.

Abstract

Four human Lactobacillus acidophilus strains were tested for their ability to adhere onto human enterocyte like Caco-2 cells in culture. The LA 1 strain exhibited a high calcium independent adhesive property. This adhesion onto Caco-2 cells required a proteinaceous adhesion promoting factor, which was present in the spent bacterial broth culture supernatant. LA 1 strain also strongly bound to the mucus secreted by the homogeneous cultured human goblet cell line HT29-MTX. The inhibitory effect of LA 1 organisms against Caco-2 cell adhesion and cell invasion by a large variety of diarrhoeagenic bacteria was investigated. As a result, the following dose dependent inhibitions were obtained: (a) against the cell association of enterotoxigenic, diffusely adhering and enteropathogenic Escherichia coli, and Salmonella typhimurium; (b) against the cell invasion by enteropathogenic Escherichia coli, Yersinia pseudotuberculosis, and Salmonella typhimurium. Incubations of L acidophilus LA 1 before and together with enterovirulent E coli were more effective than incubation after infection by E coli.

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References

Louvard D, Kedinger M, Hauri H . The differentiating intestinal epithelial cell: establishment and maintenance of functions through interactions between cellular structures. Annu Rev Cell Biol. 1992; 8:157-95. DOI: 10.1146/annurev.cb.08.110192.001105. View

Okhuysen P, Ericsson C . Travelers' diarrhea. Prevention and treatment. Med Clin North Am. 1992; 76(6):1357-73. DOI: 10.1016/s0025-7125(16)30291-7. View

Reid G, Cook R, Bruce A . Examination of strains of lactobacilli for properties that may influence bacterial interference in the urinary tract. J Urol. 1987; 138(2):330-5. DOI: 10.1016/s0022-5347(17)43137-5. View

Isberg R, Voorhis D, Falkow S . Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells. Cell. 1987; 50(5):769-78. DOI: 10.1016/0092-8674(87)90335-7. View

Gaillard J, Berche P, Mounier J, Richard S, Sansonetti P . In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun. 1987; 55(11):2822-9. PMC: 259983. DOI: 10.1128/iai.55.11.2822-2829.1987. View

Bilge S, CLAUSEN C, Lau W, Moseley S . Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol. 1989; 171(8):4281-9. PMC: 210202. DOI: 10.1128/jb.171.8.4281-4289.1989. View

Neeser J, Chambaz A, Golliard M, Fryder V, Kolodziejczyk E . Adhesion of colonization factor antigen II-positive enterotoxigenic Escherichia coli strains to human enterocytelike differentiated HT-29 cells: a basis for host-pathogen interactions in the gut. Infect Immun. 1989; 57(12):3727-34. PMC: 259897. DOI: 10.1128/iai.57.12.3727-3734.1989. View

Conway P, Kjelleberg S . Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J Gen Microbiol. 1989; 135(5):1175-86. DOI: 10.1099/00221287-135-5-1175. View

Darfeuille-Michaud A, Aubel D, Chauviere G, Rich C, Bourges M, Servin A . Adhesion of enterotoxigenic Escherichia coli to the human colon carcinoma cell line Caco-2 in culture. Infect Immun. 1990; 58(4):893-902. PMC: 258557. DOI: 10.1128/iai.58.4.893-902.1990. View

10.

Lesuffleur T, Barbat A, Dussaulx E, ZWEIBAUM A . Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Res. 1990; 50(19):6334-43. View

11.

Reid G, Bruce A, McGroarty J, Cheng K, Costerton J . Is there a role for lactobacilli in prevention of urogenital and intestinal infections?. Clin Microbiol Rev. 1990; 3(4):335-44. PMC: 358167. DOI: 10.1128/CMR.3.4.335. View

12.

Finlay B, Falkow S . Salmonella interactions with polarized human intestinal Caco-2 epithelial cells. J Infect Dis. 1990; 162(5):1096-106. DOI: 10.1093/infdis/162.5.1096. View

13.

Giron J, Jones T, Zarate L, Fry J, Frankel G, Moseley S . Diffuse-adhering Escherichia coli (DAEC) as a putative cause of diarrhea in Mayan children in Mexico. J Infect Dis. 1991; 163(3):507-13. DOI: 10.1093/infdis/163.3.507. View

14.

Aubel D, Darfeuille-Michaud A, Joly B . New adhesive factor (antigen 8786) on a human enterotoxigenic Escherichia coli O117:H4 strain isolated in Africa. Infect Immun. 1991; 59(4):1290-9. PMC: 257841. DOI: 10.1128/iai.59.4.1290-1299.1991. View

15.

Cravioto A, Tello A, Villafan H, Ruiz J, Del Vedovo S, Neeser J . Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk. J Infect Dis. 1991; 163(6):1247-55. DOI: 10.1093/infdis/163.6.1247. View

16.

Isolauri E, Juntunen M, Rautanen T, Sillanaukee P, Koivula T . A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics. 1991; 88(1):90-7. View

17.

Fogh J, FOGH J, Orfeo T . One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst. 1977; 59(1):221-6. DOI: 10.1093/jnci/59.1.221. View

18.

Evans D, Evans Jr D, Clegg S, Pauley J . Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979; 25(2):738-48. PMC: 414505. DOI: 10.1128/iai.25.2.738-748.1979. View

19.

Kleeman E, Klaenhammer T . Adherence of Lactobacillus species to human fetal intestinal cells. J Dairy Sci. 1982; 65(11):2063-9. DOI: 10.3168/jds.S0022-0302(82)82462-4. View

20.

Conway P, Gorbach S, Goldin B . Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J Dairy Sci. 1987; 70(1):1-12. DOI: 10.3168/jds.S0022-0302(87)79974-3. View