The Fas Operon of Rhodococcus Fascians Encodes New Genes Required for Efficient Fasciation of Host Plants

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 May 1

PMID 8169198

Citations 27

Authors

M Crespi

D Vereecke

W Temmerman

M Van Montagu

J Desomer

Affiliations

Soon will be listed here.

Abstract

Three virulence loci (fas, att, and hyp) of Rhodococcus fascians D188 have been identified on a 200-kb conjugative linear plasmid (pFiD188). The fas locus was delimited to a 6.5-kb DNA fragment by insertion mutagenesis, single homologous disruptive recombination, and in trans complementation of different avirulent insertion mutants. The locus is arranged as a large operon containing six open reading frames whose expression is specifically induced during the interaction with host plants. One predicted protein is homologous to P-450 cytochromes from actinomycetes. The putative ferredoxin component is of a novel type containing additional domains homologous to transketolases from chemoautotrophic, photosynthetic, and methylotrophic microorganisms. Genetic analysis revealed that fas encodes, in addition to the previously identified ipt, at least two new genes that are involved in fasciation development, one of which is only required on older tobacco plants.

Citing Articles

Biochemical and Structural Aspects of Cytokinin Biosynthesis and Degradation in Bacteria.

Frebortova J, Frebort I Microorganisms. 2021; 9(6).

PMID: 34208724 PMC: 8234997. DOI: 10.3390/microorganisms9061314.

Bacterial terpenome.

Rudolf J, Alsup T, Xu B, Li Z Nat Prod Rep. 2020; 38(5):905-980.

PMID: 33169126 PMC: 8107197. DOI: 10.1039/d0np00066c.

Comment on "Evolutionary transitions between beneficial and phytopathogenic challenge disease management".

Randall J, Stamler R, Kallsen C, Fichtner E, Heerema R, Cooke P Elife. 2018; 7.

PMID: 29737967 PMC: 5951677. DOI: 10.7554/eLife.35272.

Evolutionary transitions between beneficial and phytopathogenic challenge disease management.

Savory E, Fuller S, Weisberg A, Thomas W, Gordon M, Stevens D Elife. 2017; 6.

PMID: 29231813 PMC: 5726852. DOI: 10.7554/eLife.30925.

RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta.

Wang Z, Yan N, Wang Z, Zhang X, Zhang J, Xue H Plant Mol Biol. 2017; 95(6):533-547.

PMID: 29076026 DOI: 10.1007/s11103-017-0658-9.

References

Akiyoshi D, Regier D, Gordon M . Cytokinin production by Agrobacterium and Pseudomonas spp. J Bacteriol. 1987; 169(9):4242-8. PMC: 213736. DOI: 10.1128/jb.169.9.4242-4248.1987. View

Matthews D, VAN Etten H . Detoxification of the phytoalexin pisatin by a fungal cytochrome P-450. Arch Biochem Biophys. 1983; 224(2):494-505. DOI: 10.1016/0003-9861(83)90237-0. View

Klambt D, THIES G, Skoog F . Isolation of cytokinins from Corynebacterium fascians. Proc Natl Acad Sci U S A. 1966; 56(1):52-9. PMC: 285673. DOI: 10.1073/pnas.56.1.52. View

Janowicz Z, Eckart M, Drewke C, Roggenkamp R, Hollenberg C, Maat J . Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha. Nucleic Acids Res. 1985; 13(9):3043-62. PMC: 341220. DOI: 10.1093/nar/13.9.3043. View

Chen J, Gibson J, McCue L, Tabita F . Identification, expression, and deduced primary structure of transketolase and other enzymes encoded within the form II CO2 fixation operon of Rhodobacter sphaeroides. J Biol Chem. 1991; 266(30):20447-52. View

Omer C, Lenstra R, Litle P, Dean C, Tepperman J, Leto K . Genes for two herbicide-inducible cytochromes P-450 from Streptomyces griseolus. J Bacteriol. 1990; 172(6):3335-45. PMC: 209144. DOI: 10.1128/jb.172.6.3335-3345.1990. View

Casadaban M, Cohen S . Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980; 138(2):179-207. DOI: 10.1016/0022-2836(80)90283-1. View

Scarbrough E, Armstrong D, Skoog F, Frihart C, LEONARD N . Isolation of cis-Zeatin from Corynebacterium fascians Cultures. Proc Natl Acad Sci U S A. 1973; 70(12 Pt 1-2):3825-9. PMC: 427337. DOI: 10.1073/pnas.70.12.3825. View

Hoitink C, Woudt L, Turenhout J, van de Kamp M, Canters G . Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis. Gene. 1990; 90(1):15-20. DOI: 10.1016/0378-1119(90)90434-s. View

10.

Desomer J, Vereecke D, Crespi M, Van Montagu M . The plasmid-encoded chloramphenicol-resistance protein of Rhodococcus fascians is homologous to the transmembrane tetracycline efflux proteins. Mol Microbiol. 1992; 6(16):2377-85. DOI: 10.1111/j.1365-2958.1992.tb01412.x. View

11.

Haydock S, Dowson J, Dhillon N, Roberts G, Cortes J, Leadlay P . Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol Gen Genet. 1991; 230(1-2):120-8. DOI: 10.1007/BF00290659. View

12.

Jefferson R, Burgess S, Hirsh D . beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A. 1986; 83(22):8447-51. PMC: 386947. DOI: 10.1073/pnas.83.22.8447. View

13.

Brutlag D, Dautricourt J, Maulik S, Relph J . Improved sensitivity of biological sequence database searches. Comput Appl Biosci. 1990; 6(3):237-45. DOI: 10.1093/bioinformatics/6.3.237. View

14.

ODonnell M, Rowe B, Lawson N, Horton A, Gyde O, Barnett A . Placebo-controlled trial of lisinopril in normotensive diabetic patients with incipient nephropathy. J Hum Hypertens. 1993; 7(4):327-32. View

15.

Stassi D, Donadio S, Staver M, Katz L . Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. J Bacteriol. 1993; 175(1):182-9. PMC: 196112. DOI: 10.1128/jb.175.1.182-189.1993. View

16.

Desomer J, DHaese P, Van Montagu M . Conjugative transfer of cadmium resistance plasmids in Rhodococcus fascians strains. J Bacteriol. 1988; 170(5):2401-5. PMC: 211139. DOI: 10.1128/jb.170.5.2401-2405.1988. View

17.

Horii M, Ishizaki T, Paik S, Manome T, Murooka Y . An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp. J Bacteriol. 1990; 172(7):3644-53. PMC: 213338. DOI: 10.1128/jb.172.7.3644-3653.1990. View

18.

Desomer J, DHaese P, Montagu M . Transformation of Rhodococcus fascians by High-Voltage Electroporation and Development of R. fascians Cloning Vectors. Appl Environ Microbiol. 1990; 56(9):2818-25. PMC: 184849. DOI: 10.1128/aem.56.9.2818-2825.1990. View

19.

Shimoda N, Nagamine J, Usami S, Katayama M, Sakagami Y, Machida Y . Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides. Proc Natl Acad Sci U S A. 1990; 87(17):6684-8. PMC: 54601. DOI: 10.1073/pnas.87.17.6684. View

20.

Murai N, Skoog F, Doyle M, Hanson R . Relationships between cytokinin production, presence of plasmids, and fasciation caused by strains of Corynebacterium fascians. Proc Natl Acad Sci U S A. 1980; 77(1):619-23. PMC: 348326. DOI: 10.1073/pnas.77.1.619. View