Isolation of Plasmid Deoxyribonucleic Acid from Pseudomonas Putida

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 Apr 1

PMID 816778

Citations 18

Authors

S Palchaudhuri

A Chakrabarty

Affiliations

Soon will be listed here.

Abstract

Conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of Pseudomonas putida containing degradative plasmids (CAM, SAL, OCT, etc.) have been defined. These degradative plasmids could not be isolated by the usual procedure, whereas RP1, an R factor of the P group, present in the isogenic strain of P. putida, was isolated equally well by either the usual procedure or the modified procedure. Characterization by electron microscopy of RP1 deoxyribonucleic acid confirmed the molecular weight (about 40 X 10(6)) previously determined by sucrose gradient centrifugation.

Citing Articles

Indicator technique for antimetabolic toxin production by phytopathogenic species of pseudomonas.

Gasson M Appl Environ Microbiol. 1980; 39(1):25-9.

PMID: 16345492 PMC: 291278. DOI: 10.1128/aem.39.1.25-29.1980.

Plasmid control of 6-aminohexanoic acid cyclic dimer degradation enzymes of Flavobacterium sp. KI72.

Negoro S, Shinagawa H, Nakata A, Kinoshita S, Hatozaki T, Okada H J Bacteriol. 1980; 143(1):238-45.

PMID: 7400094 PMC: 294219. DOI: 10.1128/jb.143.1.238-245.1980.

In vivo genesis of a transposon carrying the histidine genes of Escherichia coli K-12.

Palchaudhuri S, Lakshmi T, Judge M, Murthy J Mol Gen Genet. 1984; 193(1):172-8.

PMID: 6318049 DOI: 10.1007/BF00327433.

Naphthalene plasmids in pseudomonads.

Connors M, BARNSLEY E J Bacteriol. 1982; 149(3):1096-101.

PMID: 6277849 PMC: 216500. DOI: 10.1128/jb.149.3.1096-1101.1982.

Role of nucleases in the isolation of plasmid deoxyribonucleic acid from Pseudomonas cepacia 4G9.

Williams J, Yeggy J, Markovetz A J Bacteriol. 1980; 143(2):1057-9.

PMID: 6259115 PMC: 294418. DOI: 10.1128/jb.143.2.1057-1059.1980.

References

Novick R, Maas W . Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli. J Bacteriol. 1961; 81:236-40. PMC: 278992. DOI: 10.1128/jb.81.2.236-240.1961. View

Cohen S, Miller C . Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol. 1970; 50(3):671-87. DOI: 10.1016/0022-2836(70)90092-6. View

Grinsted J, Saunders J, Ingram L, Sykes R, Richmond M . Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972; 110(2):529-37. PMC: 247445. DOI: 10.1128/jb.110.2.529-537.1972. View

Chakrabarty A . Genetic basis of the biodegradation of salicylate in Pseudomonas. J Bacteriol. 1972; 112(2):815-23. PMC: 251491. DOI: 10.1128/jb.112.2.815-823.1972. View

Pemberton J, Clark A . Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973; 114(1):424-33. PMC: 251781. DOI: 10.1128/jb.114.1.424-433.1973. View

Guerry P, Leblanc D, Falkow S . General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973; 116(2):1064-6. PMC: 285495. DOI: 10.1128/jb.116.2.1064-1066.1973. View

Palchaudhuri S, Mazaitis A, Maas W, KLEINSCHMIDT A . Characterization by electron microscopy of fused F-prime factors in Escherichia coli. Proc Natl Acad Sci U S A. 1972; 69(7):1873-6. PMC: 426822. DOI: 10.1073/pnas.69.7.1873. View

Williams P, Murray K . Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974; 120(1):416-23. PMC: 245778. DOI: 10.1128/jb.120.1.416-423.1974. View

Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View

10.

Palchoudhury S, Iyer V . Compatibility between two F' factors in an Escherichia coli strain bearing a chromosomal mutation affecting DNA synthesis. J Mol Biol. 1971; 57(2):319-33. DOI: 10.1016/0022-2836(71)90349-4. View

11.

Fitzgerald T, Miller J, SYKES J . Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975; 11(5):1141-6. PMC: 415189. DOI: 10.1128/iai.11.5.1141-1146.1975. View

12.

Kline B, Miller J . Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation. J Bacteriol. 1975; 121(1):165-72. PMC: 285627. DOI: 10.1128/jb.121.1.165-172.1975. View

13.

Radloff R, BAUER W, Vinograd J . A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967; 57(5):1514-21. PMC: 224502. DOI: 10.1073/pnas.57.5.1514. View