Identification of Conserved Domains in the Delta 12 Desaturases of Cyanobacteria

Overview

Journal Plant Mol Biol

Date 1994 Feb 1

PMID 8155883

Citations 9

Authors

T Sakamoto

H Wada

I Nishida

M Ohmori

N Murata

Affiliations

Soon will be listed here.

Abstract

Cyanobacterial genes for enzymes that desaturate fatty acids at the delta 12 position, designated desA, were isolated from Synechocystis PCC6714, Synechococcus PCC7002 and Anabaena variabilis by cross-hybridization with a DNA probe derived from the desA gene of Synechocystis PCC6803. The genes of Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis encode proteins of 349, 347 and 350 amino acid residues, respectively. The transformation of Synechococcus PCC7942 with the desA genes from Synechocystis PCC6714, Synechococcus PCC7002 and A. variabilis was associated with the ability to introduce a second double bond at the delta 12 position of fatty acids. The amino acid sequence of the products of the desA genes revealed the presence of four conserved domains. Since one of the conserved domains was also found in the amino acid sequences of omega 3 desaturases of Brassica napus and mung bean, this domain may play an essential role in the introduction of a double bond into fatty acids bound to membrane lipids.

Citing Articles

The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species.

Dailey F, McGraw J, Jensen B, Bishop S, Lokken J, Dorff K Appl Environ Microbiol. 2015; 82(1):218-31.

PMID: 26497452 PMC: 4702627. DOI: 10.1128/AEM.02266-15.

A desaturase gene involved in the formation of 1,14-nonadecadiene in Synechococcus sp. strain PCC 7002.

Mendez-Perez D, Herman N, Pfleger B Appl Environ Microbiol. 2014; 80(19):6073-9.

PMID: 25063658 PMC: 4178698. DOI: 10.1128/AEM.01615-14.

The initiation ketosynthase (FabH) is the sole rate-limiting enzyme of the fatty acid synthase of Synechococcus sp. PCC 7002.

Kuo J, Khosla C Metab Eng. 2014; 22:53-9.

PMID: 24395007 PMC: 4040180. DOI: 10.1016/j.ymben.2013.12.008.

Identification and functional characterization of two Δ12-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens.

Chodok P, Eiamsa-ard P, Cove D, Quatrano R, Kaewsuwan S J Ind Microbiol Biotechnol. 2013; 40(8):901-13.

PMID: 23702573 DOI: 10.1007/s10295-013-1285-3.

A novel Delta9 acyl-lipid desaturase, DesC2, from cyanobacteria acts on fatty acids esterified to the sn-2 position of glycerolipids.

Chintalapati S, Prakash J, Gupta P, Ohtani S, Suzuki I, Sakamoto T Biochem J. 2006; 398(2):207-14.

PMID: 16689682 PMC: 1550309. DOI: 10.1042/BJ20060039.

References

Stukey J, McDonough V, Martin C . The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene. J Biol Chem. 1990; 265(33):20144-9. View

Arondel V, Lemieux B, Hwang I, Gibson S, Goodman H, Somerville C . Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science. 1992; 258(5086):1353-5. DOI: 10.1126/science.1455229. View

Reddy A, Nuccio M, Gross L, Thomas T . Isolation of a delta 6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp. strain PCC 7120. Plant Mol Biol. 1993; 22(2):293-300. DOI: 10.1007/BF00014936. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Wada H, Gombos Z, Murata N . Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature. 1990; 347(6289):200-3. DOI: 10.1038/347200a0. View

Thiede M, Ozols J, STRITTMATTER P . Construction and sequence of cDNA for rat liver stearyl coenzyme A desaturase. J Biol Chem. 1986; 261(28):13230-5. View

Wada H, Schmidt H, Heinz E, Murata N . In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. J Bacteriol. 1993; 175(2):544-7. PMC: 196170. DOI: 10.1128/jb.175.2.544-547.1993. View

Schmidt H, Heinz E . Involvement of ferredoxin in desaturation of lipid-bound oleate in chloroplasts. Plant Physiol. 1990; 94(1):214-20. PMC: 1077212. DOI: 10.1104/pp.94.1.214. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

10.

G Williams J, Szalay A . Stable integration of foreign DNA into the chromosome of the cyanobacterium Synechococcus R2. Gene. 1983; 24(1):37-51. DOI: 10.1016/0378-1119(83)90129-4. View

11.

Haselkorn R . Genetic systems in cyanobacteria. Methods Enzymol. 1991; 204:418-30. DOI: 10.1016/0076-6879(91)04022-g. View

12.

STANIER R, Kunisawa R, Mandel M, COHEN-BAZIRE G . Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971; 35(2):171-205. PMC: 378380. DOI: 10.1128/br.35.2.171-205.1971. View

13.

Kuhlemeier C, Thomas A, van der Ende A, van Leen R, Borrias W, van den Hondel C . A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2. Plasmid. 1983; 10(2):156-63. DOI: 10.1016/0147-619x(83)90068-9. View