Oxidative Phosphorylation During Glycollate Metabolism in Mitochondria from Phototrophic Euglena Gracilis

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Sep 1

PMID 813630

Citations 13

Authors

N Collins

R H Brown

M J Merrett

Affiliations

Soon will be listed here.

Abstract

Mitochondria were isolated by gradient centrifugation on linear sucrose gradients from broken cell suspensions of phototrophically grown Euglena gracilis. An antimycin A-sensitive but rotenone-insensitive glycollate-dependent oxygen uptake was demonstrated in isolated mitochondria. The partial reactions of glycollate-cytochrome c oxidoreductase and cytochrome c oxidase were demonstrated by using Euglena cytochrome c as exogenous electron acceptor/donor. Isolated mitochondria contain glycollate dehydrogenase and glyoxylate-glutamate aminotransferase and oxidize exogenous glycine. A P:O ratio of 1.7 was obtained for glycollate oxidation, consistent with glycollate electrons entering the Euglena respiratory chain at the flavoprotein level. The significance of these results is discussed in relation to photorespiration in algae.

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