Staphylococcin 1580 is Identical to the Lantibiotic Epidermin: Implications for the Nature of Bacteriocins from Gram-positive Bacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1994 Feb 1

PMID 8135526

Citations 10

Authors

H G Sahl

Affiliations

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Abstract

Staphylococcin 1580 was purified to homogeneity from culture supernatants of Staphylococcus epidermidis 1580 by means of adsorption to XAD 2, cation exchange chromatography, and high-performance liquid chromatography on reversed-phase C18. The purified active substance migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent M(r) of approximately 2,000. Amino acid analysis, mass determination (2,165 Da) and N-terminal sequencing (Ile-Ala-Xaa-Lys-Phe-Ile-Xaa-Xaa-Pro-Gly-Xaa-Ala-Lys-block) demonstrated that staphylococcin 1580 is identical to epidermin, a lanthionine-containing antibiotic peptide (lantibiotic).

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References

Lachowicz T, WALCZAK Z . Purification and properties of staphylococcin A. Arch Immunol Ther Exp (Warsz). 1968; 16(6):855-63. View

Mulders J, Boerrigter I, Rollema H, Siezen R, de Vos W . Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. Eur J Biochem. 1991; 201(3):581-4. DOI: 10.1111/j.1432-1033.1991.tb16317.x. View

Gagliano V, HINSDILL R . Characterization of a Staphylococcus aureus bacteriocin. J Bacteriol. 1970; 104(1):117-25. PMC: 248190. DOI: 10.1128/jb.104.1.117-125.1970. View

Jetten A, Vogels G, de Windt F . Production and purification of a Staphylococcus epidermidis bacteriocin. J Bacteriol. 1972; 112(1):235-42. PMC: 251403. DOI: 10.1128/jb.112.1.235-242.1972. View

Jetten A, Vogels G . Nature and properties of a Staphylococcus epidermidis bacteriocin. J Bacteriol. 1972; 112(1):243-50. PMC: 251404. DOI: 10.1128/jb.112.1.243-250.1972. View

Jetten A, Vogels G . Effects of colicin A and staphylococcin 1580 on amino acid uptake into membrane vesicles of Escherichia coli and staphylococcus aureus. Biochim Biophys Acta. 1973; 311(4):483-95. DOI: 10.1016/0005-2736(73)90124-7. View

Jetten A, Vogels G . Mode of action of a Staphylococcus epidermidis bacteriocin. Antimicrob Agents Chemother. 1972; 2(6):456-63. PMC: 444339. DOI: 10.1128/AAC.2.6.456. View

Hale E, HINSDILL R . Characterization of a bacteriocin from Staphylococcus aureus strain 462. Antimicrob Agents Chemother. 1973; 4(6):634-40. PMC: 444609. DOI: 10.1128/AAC.4.6.634. View

Tagg J, Dajani A, Wannamaker L . Bacteriocins of gram-positive bacteria. Bacteriol Rev. 1976; 40(3):722-56. PMC: 413978. DOI: 10.1128/br.40.3.722-756.1976. View

10.

Rogolsky M, Wiley B . Production and properties of a staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis. Infect Immun. 1977; 15(3):726-32. PMC: 421430. DOI: 10.1128/iai.15.3.726-732.1977. View

11.

Weerkamp A, Geerts W, Vogels G . Energy requirements for the action of staphylococcin 1580 in Staphyloccus aureus. Biochim Biophys Acta. 1978; 539(3):372-85. DOI: 10.1016/0304-4165(78)90041-7. View

12.

Sahl H, BRANDIS H . Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis. J Gen Microbiol. 1981; 127(2):377-84. DOI: 10.1099/00221287-127-2-377. View

13.

Sahl H, BRANDIS H . Mode of action of the staphylococcin-like peptide Pep 5 and culture conditions effecting its activity. Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1982; 252(2):166-75. View

14.

Konisky J . Colicins and other bacteriocins with established modes of action. Annu Rev Microbiol. 1982; 36:125-44. DOI: 10.1146/annurev.mi.36.100182.001013. View

15.

Nakamura T, Yamazaki N, Taniguchi H, Fujimura S . Production, purification, and properties of a bacteriocin from Staphylococcus aureus isolated from saliva. Infect Immun. 1983; 39(2):609-14. PMC: 347995. DOI: 10.1128/iai.39.2.609-614.1983. View

16.

Sahl H . Influence of the staphylococcinlike peptide Pep 5 on membrane potential of bacterial cells and cytoplasmic membrane vesicles. J Bacteriol. 1985; 162(2):833-6. PMC: 218930. DOI: 10.1128/jb.162.2.833-836.1985. View

17.

Bierbaum G, Sahl H . Induction of autolysis of staphylococci by the basic peptide antibiotics Pep 5 and nisin and their influence on the activity of autolytic enzymes. Arch Microbiol. 1985; 141(3):249-54. DOI: 10.1007/BF00408067. View

18.

Sahl H, Grossgarten M, Widger W, Cramer W, BRANDIS H . Structural similarities of the staphylococcin-like peptide Pep-5 to the peptide antibiotic nisin. Antimicrob Agents Chemother. 1985; 27(5):836-40. PMC: 180162. DOI: 10.1128/AAC.27.5.836. View

19.

Ruhr E, Sahl H . Mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles. Antimicrob Agents Chemother. 1985; 27(5):841-5. PMC: 180163. DOI: 10.1128/AAC.27.5.841. View

20.

Allgaier H, Jung G, Werner R, Schneider U, Zahner H . Epidermin: sequencing of a heterodetic tetracyclic 21-peptide amide antibiotic. Eur J Biochem. 1986; 160(1):9-22. DOI: 10.1111/j.1432-1033.1986.tb09933.x. View